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AB275555

重组人DNase I蛋白(Active)

Recombinant human DNase I protein (Active)

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Recombinant human DNase I protein (Active) is a Human Fragment protein, in the 19 to 259 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS, WB.

查看别名

DNL1, DRNI, DNASE1, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I

2 Images
Functional Studies - Recombinant human DNase I protein (Active) (AB275555)
  • FuncS

Supplier Data

Functional Studies - Recombinant human DNase I protein (Active) (AB275555)

The binding activity of DNase I and ACTb.

DNase I were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DNase I pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately.

SDS-PAGE - Recombinant human DNase I protein (Active) (AB275555)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human DNase I protein (Active) (AB275555)

SDS-PAGE - Recombinant human DNase I protein (Active) (ab275555).

关键信息

纯度

>90% SDS-PAGE

nan

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

FuncS, SDS-PAGE, WB

applications

生物活性

Yes

生物学活性

Active

访问

P24855

不含动物源

No

不含载体蛋白

No

种属

Human

复溶

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

存储溶液

pH: 8 Constituents: PBS, 82.8% Trehalose, 0.17% Sodium-N-Lauroylsarcosinate

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

  • 5
  • 1
Assay Kits

AB234056

DNase I Assay Kit (Fluorometric)

Functional Studies - DNase I Assay Kit (Fluorometric) (AB234056)

  • 4
  • 1
Primary Antibodies

AB309566

Anti-DNase I antibody [EPR25941-19] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-DNase I antibody [EPR25941-19] - BSA and Azide free (Detector) (AB309566)

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  • 0
Proteins & Peptides

AB238220

Recombinant Human DNase gamma protein (Tagged)

SDS-PAGE - Recombinant Human DNase gamma protein (Tagged) (AB238220)

  • 0
  • 0
Assay Kits

AB315786

Human DNase I ELISA Kit

SimpleStep

Sandwich ELISA - Human DNase I ELISA Kit (AB315786)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Using recombinant protein.</p>" } } }
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序列信息

[{"linker":null,"sequence":"GAVSLKIAAFNIQTFGETKMSNATLVSYIVQILSRYDIALVQEVRDSHLTAVGKLLDNLNQDAPDTYHYVVSEPLGRNSYKERYLFVYRPDQVSAVDSYYYDDGCEPCGNDTFNREPAIVRFFSRFTEVREFAIVPLHAAPGDAVAEIDALYDVYLDVQEKWGLEDVMLMGDFNAGCSYVRPSQWSSIRLWTSPTFQWLIPDSADTTATPTHCAYDRIVVAGMLLRGAVVPDSALPFNFQA","proteinLength":"Fragment","predictedMolecularWeight":"31 kDa","actualMolecularWeight":null,"aminoAcidEnd":259,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P24855","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Lyophilized
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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常规信息

功能

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed : 11241278, PubMed : 2251263, PubMed : 2277032). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (PubMed : 11241278). Binds specifically to G-actin and blocks actin polymerization (By similarity). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).

序列相似性

Belongs to the DNase I family.

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产品实验方案

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靶点信息

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed : 11241278, PubMed : 2251263, PubMed : 2277032). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (PubMed : 11241278). Binds specifically to G-actin and blocks actin polymerization (By similarity). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
See full target information DNASE1
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