重组人DNA Polymerase lambda蛋白(ab113399)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
描述
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产品名称
重组人DNA Polymerase lambda蛋白 -
纯度
> 90 % SDS-PAGE.
Conventional chromatography techniques (anion exchange followed by gel filtration with 20mM Tris pH 7.5, 2mM EDTA). -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MGSSHHHHHHSSGLVPRGSHMLMHHQKYLQRFLGGKREKKQKEACSIPGI GKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQMWYQQ GFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQKAAQA FNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSLRQEG FLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLY FTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRVLPTPT EKDVFRLLGLPYREPAERDW -
预测分子量
36 kDa -
氨基酸
1 to 300 -
标签
His tag N-Terminus -
额外的序列信息
The protein sequence refers to isoform 2 and contains MGSSHHHHHHSSGLVPRGSH sequence at N-Terminus for the His tag.
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描述
重组人DNA Polymerase lambda/Polk蛋白
相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab113399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Mass Spectrometry
SDS-PAGE
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质谱法
MALDI-TOF -
形式
Liquid -
补充说明
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
常规信息
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别名
- BETA N
- BETAN
- DNA directed DNA polymerase lambda
see all -
功能
Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. -
组织特异性
Expressed in a number of tissues. Abundant in testis. -
序列相似性
Belongs to the DNA polymerase type-X family.
Contains 1 BRCT domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human DNA Polymerase lambda protein (ab113399)
15% SDS-PAGE of DNA Polymerase lambda protein: (1) MW ladder, (2) protein loaded 3ug
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab113399 尚未被引用在任何文献中。