重组人DNA Polymerase Kappa/POLK蛋白(ab176956)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, SDS-PAGE, WB, Functional Studies
描述
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产品名称
重组人DNA Polymerase Kappa/POLK蛋白 -
纯度
> 90 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFY GNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLS NTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRA AMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEA YLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISP LLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFR IEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLP IRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISL GLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKER LKGRTVTIKLKNVNFEVKTRASTVSSVVSTAEEIFAIAKELLKTEIDADF PHPLRLRLMGVRISSFPNEEDRKHQQRSIIGFLQAGNQALSATECTLEKT DKDKFVKPLE -
预测分子量
64 kDa -
氨基酸
1 to 560 -
标签
His tag C-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab176956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
ELISA
SDS-PAGE
Western blot
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C or -80°C.
pH: 6
Constituents: 1.17% Sodium chloride, 0.14% Sodium phosphate, 50% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- DinB homolog 1 (E. coli)
- DINB protein
- DINB1
see all -
功能
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. -
组织特异性
Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes. -
序列相似性
Belongs to the DNA polymerase type-Y family.
Contains 2 UBZ-type zinc fingers.
Contains 1 umuC domain. -
结构域
The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity. -
细胞定位
Nucleus. Detected throughout the nucleus and at replication foci. - Information by UniProt
图片
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Functional Studies - Recombinant human DNA Polymerase Kappa/POLK protein (ab176956)
DNA polymerizing activity of ab176956
50 mM Tris-HCl pH 8.0, 2 mM MgCl2, 1 mM DTT, 0.1 mg / ml BSA, 0.1 mM dNTP, 100 nM primer/template(13-mer/30-mer), DNA polymerase kappa/POLK at 13~50 ng/25μl (lanes 1,2, and 3 respectiviely) per assay, incubated at 37℃ for 10 min. -
SDS-PAGE - Recombinant human DNA Polymerase Kappa/POLK protein (ab176956)SDS-PAGE analysis of ab196956.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab176956 尚未被引用在任何文献中。