重组人DNA Polymerase beta蛋白(ab111641)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
描述
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产品名称
重组人DNA Polymerase beta蛋白
参阅全部 DNA Polymerase beta 蛋白酶 -
纯度
> 90 % SDS-PAGE.
ab111641 is purified using conventional chromatography. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MGSSHHHHHHSSGLVPRGSHMSKRKAPQETLNGGITDMLTELANFEKNVS QAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATG KLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNED KLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGS FRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSK GETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNK NMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREP KDRSE -
预测分子量
40 kDa including tags -
氨基酸
1 to 335 -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab111641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Mass Spectrometry
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质谱法
MALDI-TOF -
形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.02% DTT, 69% Tris HCl, 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
常规信息
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别名
- DNA directed DNA polymerase beta
- DNA pol beta
- DNA polymerase beta
see all -
功能
Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. -
序列相似性
Belongs to the DNA polymerase type-X family. -
结构域
Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity. -
翻译后修饰
Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. -
细胞定位
Nucleus, Cytoplasm - Information by UniProt
图片
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SDS-PAGE - Recombinant Human DNA Polymerase beta protein (ab111641)15% SDS-PAGE analysis of ab111641 (3 µg)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab111641 尚未被引用在任何文献中。