重组人DLL4蛋白(Fc Chimera Active) (ab108557)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 0.010 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人DLL4蛋白(Fc Chimera Active)
参阅全部 DLL4 蛋白酶 -
生物活性
Inhibits adipogenesis of 3T3L-1 cells and mesenchymal stem cells (MSCs). Induces the Notch target gene HES-1 when coated on a plate at 1µg/ml.
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纯度
>= 95 % SDS-PAGE.
Purified using affinity chromatography. -
内毒素水平
< 0.010 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
80 kDa including tags -
氨基酸
1 to 529 -
标签
Fc tag C-Terminus -
额外的序列信息
Signal peptide and extracellular domain of human DLL4 (aa 1-529) are fused at the C-terminus to the Fc portion of human IgG1.
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技术指标
Our Abpromise guarantee covers the use of ab108557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
补充说明
ab108557 interacts with Human Notch1 (as confirmed by Flow Cytometry).
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: PBS, 0.5% Trehalose
ab108557 is 0.2 µm filtered.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with 100µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions. Working aliquots are stable for up to 3 months when stored at -20°C.
常规信息
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别名
- AOS6
- Delta 4
- delta 4 precursor
see all -
功能
Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4. -
组织特异性
Expressed in vascular endothelium. -
序列相似性
Contains 1 DSL domain.
Contains 8 EGF-like domains. -
结构域
The Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor. -
翻译后修饰
Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. -
细胞定位
Membrane. - Information by UniProt
图片
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Functional Studies - Recombinant human DLL4 protein (Fc Chimera Active) (ab108557)Interaction of Human Notch1 with Human DLL4.
HEK293 cells transfected with a Human Notch1 or a Human GITR ligand expressing vector were incubated with 25 μg/ml of Human GITR-Fc or ab108557. Cells were stained with anti-Human IgG (Fc specific) FITC conjugate for DLL4-Fc binding. -
Functional Studies - Recombinant human DLL4 protein (Fc Chimera Active) (ab108557)Induction of Hes-1 with the treatment of recombinant Human DLL4-Fc (ab108557).
A Mouse preadpipocyte cell line, 3T3L-1, was stimulated with 5 μg/ml of Human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-Mouse Hes1 or GAPDH.
M: Marker.
Lane 1: hDLL4-Fc, 0 min.
Lane 2: hDLL4-Fc, 10 min.
Lane 3: hDLL4-Fc, 30 min.
Lane 4: hDLL4-Fc, 1 hr.
Lane 5: hDLL4-Fc, 2 hr.
Lane 6: hDLL4-Fc, 4 hr.
Lane 7: hDLL4-Fc, 8 hr.
Lane 8: hDLL4-Fc, 24 hr. -
Functional Studies - Recombinant human DLL4 protein (Fc Chimera Active) (ab108557)Adipogenesis inhibition of 3T3L-1 cells.
3T3L-1 cells (mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 cells, 3T3L-1 cells were cultured in adipogenic medium which was growth medium supplemented with 1 μM Dexamethasone, 0.5 mM IBMX, 10 μg/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-α (20 ng/ml) was added. Recombinant Human DLL4-Fc (ab108557) (5 μg/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 cells. -
Functional Studies - Recombinant human DLL4 protein (Fc Chimera Active) (ab108557)Adipogenesis inhibition of MSCs.
MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilinstreptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 μM Dexamethasone, 0.5mM IBMX, 10 μg/m lnsulin, 100 μM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-α (20 ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of ab108557 (5 μg/ml) or mCD137-Fc (5 μg/ml) in PBS for 2 hours at 37°C. Plates were then used to differentiate MSCs. -
Functional Studies - Recombinant human DLL4 protein (Fc Chimera Active) (ab108557)Adipogenesis inhibition of 3T3L-1 cells. -
Functional Studies - Recombinant human DLL4 protein (Fc Chimera Active) (ab108557)50 μg of cell lysates derived from hDLL4-Fc (ab108557) or non-treated 3T3L-1 cells, which had been either differentiated or undifferentiated, and were subjected to Western blot by using a Mouse adiponectin antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (7)
ab108557 被引用在 7 文献中.
- Tiemeijer LA et al. Engineered patterns of Notch ligands Jag1 and Dll4 elicit differential spatial control of endothelial sprouting. iScience 25:104306 (2022). PubMed: 35602952
- Bill M et al. EGFL7 Antagonizes NOTCH Signaling and Represents a Novel Therapeutic Target in Acute Myeloid Leukemia. Clin Cancer Res 26:669-678 (2020). PubMed: 31672772
- Kamalakar A et al. A non-canonical JAGGED1 signal to JAK2 mediates osteoblast commitment in cranial neural crest cells. Cell Signal 54:130-138 (2019). PubMed: 30529759
- Tiemeijer LA et al. Spatial patterning of the Notch ligand Dll4 controls endothelial sprouting in vitro. Sci Rep 8:6392 (2018). PubMed: 29686270
- Tang D et al. Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin ß1 in Keratinocyte Migration. Sci Rep 7:43926 (2017). PubMed: 28266574
- Faronato M et al. DMXL2 drives epithelial to mesenchymal transition in hormonal therapy resistant breast cancer through Notch hyper-activation. Oncotarget 6:22467-79 (2015). PubMed: 26093085
- Hale AT et al. Endothelial Kruppel-like factor 4 regulates angiogenesis and the Notch signaling pathway. J Biol Chem 289:12016-28 (2014). PubMed: 24599951
