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AB130040

重组人DIMT1L蛋白

Recombinant Human DIMT1L protein

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Recombinant Human DIMT1L protein is a Human Full Length protein, in the 1 to 313 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

查看别名

DIMT1L, HUSSY-05, DIMT1, Dimethyladenosine transferase, 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase, 18S rRNA dimethylase, DIM1 dimethyladenosine transferase 1 homolog, DIM1 dimethyladenosine transferase 1-like

1 Images
SDS-PAGE - Recombinant Human DIMT1L protein (AB130040)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human DIMT1L protein (AB130040)

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

Mass Spec, SDS-PAGE

applications

生物活性

No

访问

Q9UNQ2

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.06% EDTA, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMMPKVKSGAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIIIPEDFSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS","proteinLength":"Full Length","predictedMolecularWeight":"37.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":313,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UNQ2","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

DIMT1L also known as DIMT1 or Nsun5 is a 58 kDa protein involved in ribosomal RNA methylation. It functions as a methyltransferase introducing methyl groups at specific adenine residues in the small subunit ribosomal RNA (rRNA). The protein shows expression mainly in tissues with high rates of protein synthesis such as liver kidney and pancreas reflecting its role in ribosome biogenesis. Its widespread expression in proliferative tissues highlights its importance for cellular growth and division.
Biological function summary

The protein DIMT1L plays an important role in assembling the 40S ribosomal subunit a part of the eukaryotic 80S ribosome. As a methyltransferase this protein methylates adenine residues in rRNA influencing the ribosome's structural and functional integrity. It usually functions as a monomer not requiring large complexes to exert its effects. This methyltransferase activity is important for correct ribosome maturation which in turn affects protein synthesis capacity within cells.

Pathways

DIMT1L activity is integral to ribosome biogenesis and rRNA processing pathways. The protein interacts with components of the ribonucleoprotein complex to facilitate the final maturation steps of the 40S subunit. It associates with proteins like Nop14 which participate in similar rRNA processing pathways ensuring the fidelity of ribosome assembly. Its role in these pathways highlights the importance of ribosomal integrity for effective translation and overall cellular function.

Research links DIMT1L to certain cancers and growth defects. Aberrations in its expression or function can lead to dysregulation in ribosome production contributing to tumorigenesis. The protein sometimes connects to pathways involving tumor suppressor proteins like p53 where disruption can accelerate cancer progression. Beyond cancer other proliferative disorders might arise from alterations in DIMT1L-mediated processes highlighting the need for further exploration into its mechanistic impacts on human health.
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特殊说明

形式

Liquid

附加说明

ab130040 is purified using conventional chromatography techniques.

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常规信息

功能

Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle (PubMed : 25851604). Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity (PubMed : 25851604). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

序列相似性

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle (PubMed : 25851604). Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity (PubMed : 25851604). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).
See full target information DIMT1
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搭配使用产品

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Primary Antibodies

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Primary Antibodies

AB202064

Anti-PPCEL/PREPL antibody [EPR17751]

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Western blot - Anti-PPCEL/PREPL antibody [EPR17751] (AB202064)

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Primary Antibodies

AB220160

Anti-YTHDC2 antibody [EPR21820-49]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC2 antibody [EPR21820-49] (AB220160)

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