AB87755

重组人Dihydrofolate reductase (DHFR)蛋白

Name: 重组人Dihydrofolate reductase (DHFR)蛋白 (ab87755)| Abcam中文官网
Brand: Abcam Limited
SKU: AB87755
Price: 3985 CNY
Availability: InStock

Recombinant human Dihydrofolate reductase (DHFR) protein

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Recombinant human Dihydrofolate reductase (DHFR) protein is a Human Full Length protein, in the 1 to 187 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

查看别名

Dihydrofolate reductase, DHFR

1 Images

SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Dihydrofolate reductase (DHFR) protein (AB87755)

ab87755 at 3 ug visualised by SDS-PAGE. Observed band size is approximately 27 kDa.

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

应用

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

Specific activity is > 2000pmol/min/μg, and is defined as the amount of enzyme that converts 1.0pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25°C.

访问

P00374

不含动物源

不含载体蛋白

种属

Human

存储溶液

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND","proteinLength":"Full Length","predictedMolecularWeight":"23.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":187,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00374","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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性能和储存信息

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

True

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特殊说明

形式

Liquid

附加说明

purified by using conventional chromatography techniques.

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常规信息

功能

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

序列相似性

Belongs to the dihydrofolate reductase family.

亚细胞定位

Mitochondrion

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information Dihydrofolate reductase

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重组人Dihydrofolate reductase (DHFR)蛋白

Recombinant human Dihydrofolate reductase (DHFR) protein

SDS-PAGE - Recombinant human Dihydrofolate reductase (DHFR) protein (AB87755)

关键信息

纯度

表达系统

标签

应用

生物活性

生物学活性

访问

不含动物源

不含载体蛋白

种属

存储溶液