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AB163883

重组人 Deptor 蛋白 (GST tag N-Terminus)

Recombinant Human Deptor protein (GST tag N-Terminus)

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Recombinant Human Deptor protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 409 aa range, expressed in Wheat germ, suitable for ELISA, WB.

查看别名

DEPDC6, DEPTOR, DEP domain-containing mTOR-interacting protein, hDEPTOR, DEP domain-containing protein 6

1 Images
SDS-PAGE - Recombinant Human Deptor protein (GST tag N-Terminus) (AB163883)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Deptor protein (GST tag N-Terminus) (AB163883)

ab163883 on a 12.5% SDS-PAGE stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

ELISA, WB

applications

生物活性

No

访问

Q8TB45

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MEEGGSTGSAGSDSSTSGSGGAQQRELERMAEVLVTGEQLRLRLHEEKVIKDRRHHLKTYPNCFVAKELIDWLIEHKEASDRETAIKLMQKLADRGIIHHVCDEHKEFKDVKLFYRFRKDDGTFPLDNEVKAFMRGQRLYEKLMSPENTLLQPREEEGVKYERTFMASEFLDWLVQEGEATTRKEAEQLCHRLMEHGIIQHVSSKHPFVDSNLLYQFRMNFRRRRRLMELLNEKSPSSQETHDSPFCLRKQSHDNRKSTSFMSVSPSKEIKTVSAVRRSSMSSCGSSGYFSSSPTLSSSPPVLCNPKSVLKRPVTSEELLTPGAPYARKTFTIVGDAVGWGFVVRGSKPCHIQAVDPSGPAAAAGMKVCQFVVSVNGLNVLHVDYRTVNNLILTGPRTIVMEVMEELEC","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":409,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8TB45","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

DEPTOR also known as DEP domain-containing mTOR-interacting protein functions as a subunit of the mTOR (mechanistic Target of Rapamycin) complexes. This protein has a molecular weight of approximately 48 kDa and is expressed extensively in tissues such as skeletal muscle adipose tissue and the brain. DEPTOR directly interacts with the mTOR complexes modulating their activity. Its exact mechanical role involves binding to mTOR and influencing the assembly and function of mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2).
Biological function summary

DEPTOR is important for the regulation of cell growth and metabolism. It is a component of the mTORC1 and mTORC2 two key protein complexes that control cell proliferation survival and autophagy. DEPTOR acts as an inhibitor within these complexes reducing their activity. By regulating the mTOR signaling pathway it helps balance anabolic and catabolic processes within the cell. Without proper DEPTOR function cells may grow unchecked leading to various issues including metabolic dysregulation.

Pathways

DEPTOR plays a significant role in the mTOR signaling pathway and the PI3K/AKT pathway. In the mTOR pathway DEPTOR controls the activity of mTORC1 and mTORC2 which are essential for translating signaling into cellular response. The PI3K/AKT pathway also involves DEPTOR as it relates to insulin signaling and glucose homeostasis. Proteins such as AKT and S6K work in this complex to transmit signals impacting numerous cellular activities. The dynamic balance it maintains within these pathways is critical for normal cellular function and metabolism.

DEPTOR is associated with conditions such as cancer and type 2 diabetes. In cancers its reduced expression allows for unchecked mTOR activity driving tumor growth and survival. Conversely unregulated expression can lead to increased cell apoptosis. DEPTOR's dysregulation in the context of type 2 diabetes affects glucose metabolism through interactions with the PI3K/AKT pathway. It also interacts with proteins like IRS-1 influencing insulin sensitivity and leading to metabolic disorders. DEPTOR's regulation of these pathways suggests potential therapeutic targets in these diseases.
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特殊说明

形式

Liquid

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常规信息

功能

Negative regulator of the mTORC1 and mTORC2 complexes : inhibits the protein kinase activity of MTOR, thereby inactivating both complexes (PubMed : 19446321, PubMed : 22017875, PubMed : 22017876, PubMed : 22017877, PubMed : 25936805, PubMed : 29382726, PubMed : 34519268, PubMed : 34519269). DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy (PubMed : 22017875, PubMed : 22017876, PubMed : 22017877). In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity (PubMed : 34519268, PubMed : 34519269).

翻译后修饰

Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2 (PubMed:19446321). Phosphorylated at Ser-286, Ser-287 and Ser-291 in response to mitogenic stimulation by MTOR: DEPTOR is either directly phosphorylated by MTOR or indirectly via proteins kinases that are activated by MTOR, such as CK1/CSNK1A1 (PubMed:22017875, PubMed:22017876, PubMed:22017877). Phosphorylation at Ser-286, Ser-287 and Ser-291 promotes ubiquitination by the SCF(BTRC) complex, followed by degradation (PubMed:22017875, PubMed:22017876, PubMed:22017877). Phosphorylation at Ser-235 by MAPK3/ERK1 promotes deubiquitination by USP7, enhancing its stability (PubMed:35216969). Phosphorylation at Tyr-289 by SYK impairs its interaction with MTOR, promoting mTORC1 and mTORC2 signaling (PubMed:34634301).. Ubiquitinated; leading to proteasomal degradation (PubMed:22017875, PubMed:22017876, PubMed:22017877, PubMed:30080879, PubMed:33110214). Ubiquitination by the SCF(BTRC) and SCF(FBXW11) complexes following phosphorylation at Ser-286, Ser-287 and Ser-291 by MTOR, leads to its degradation by the proteasome (PubMed:22017875, PubMed:22017876, PubMed:22017877, PubMed:33110214). Deubiquitinated by OTUB1 in response to amino acid via a non-canonical mechanism, leading to DEPTOR stability (PubMed:29382726). Deubiquitinated by USP7 following phosphorylation at Ser-235, promoting its stability (PubMed:35216969).

亚细胞定位

Lysosome membrane

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产品实验方案

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靶点信息

Negative regulator of the mTORC1 and mTORC2 complexes : inhibits the protein kinase activity of MTOR, thereby inactivating both complexes (PubMed : 19446321, PubMed : 22017875, PubMed : 22017876, PubMed : 22017877, PubMed : 25936805, PubMed : 29382726, PubMed : 34519268, PubMed : 34519269). DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy (PubMed : 22017875, PubMed : 22017876, PubMed : 22017877). In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity (PubMed : 34519268, PubMed : 34519269).
See full target information DEPTOR
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搭配使用产品

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