重组人DAP Kinase 1蛋白(ab63191)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人DAP Kinase 1蛋白
参阅全部 DAP Kinase 1 蛋白酶 -
生物活性
Specific activity: 49 nmol/min/mg.
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纯度
> 80 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
氨基酸
1 to 363
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab63191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- DAK1
- DAP K1
- DAP kinase 1
see all -
功能
Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 10 ANK repeats.
Contains 1 death domain.
Contains 1 protein kinase domain. -
翻译后修饰
Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex, leading to its degradation by the proteasome. -
细胞定位
Cytoplasm. Colocalizes with the actin filament system. - Information by UniProt
图片
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Functional Studies - Recombinant human DAP Kinase 1 protein (ab63191)The specific activity of DAP Kinase 1 (ab63191) was determined to be 42 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human DAP Kinase 1 protein (ab63191)SDS PAGE analysis of ab63191 -
SDS-PAGE - Recombinant human DAP Kinase 1 protein (ab63191)SDS PAGE analysis of ab63191 -
Functional Studies - Recombinant human DAP Kinase 1 protein (ab63191)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human DAP Kinase 1 protein (ab63191)ab63191 on SDS-PAGE. Molecular Weight: ~71 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab63191 被引用在 1 文献中.
- Jadhav VS et al. Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice. Mol Neurodegener 15:62 (2020). PubMed: 33115519