重组人Cyclin Y蛋白(Tagged) (ab174060)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB
描述
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产品名称
重组人Cyclin Y蛋白(Tagged)
参阅全部 Cyclin Y 蛋白酶 -
纯度
> 80 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
GNTTSCCVSSSPKLRRNAHSRLESYRPDTDLSREDTGCNLQHISDRENID DLNMEFNPSDHPRASTIFLSKSQTDVREKRKSLFINHHPPGQIARKYSSC STIFLDDSTVSQPNLKYTIKCVALAIYYHIKNRDPDGRMLLDIFDENLHP LSKSEVPPDYDKHNPEQKQIYRFVRTLFSAAQLTAECAIVTLVYLERLLT YAEIDICPANWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNE LERQFLELLQFNINVPSSVYAKYYFDLRSLAEANNLSFPLEPLSRERAHK LEAISRLCEDKYKDLRRSARKRSASADNLTLPRWSPAIIS -
预测分子量
65 kDa including tags -
氨基酸
2 to 341 -
标签
GST tag N-Terminus -
额外的序列信息
(NM_145012)
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab174060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.29% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
常规信息
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别名
- C10orf9
- CBCP1
- CCNX
see all -
功能
Positive regulatory subunit of the cyclin-dependent kinases CDK14/PFTK1 and CDK16. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma membrane and promoting phosphorylation of LRP6, leading to the activation of the Wnt signaling pathway. Recruits CDK16 to the plasma membrane. Isoform 3 might play a role in the activation of MYC-mediated transcription. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the cyclin family. Cyclin Y subfamily.
Contains 1 cyclin N-terminal domain. -
发展阶段
Enriched at G2/M. -
翻译后修饰
Ubiquitinated; leading to its degradation.
Heavily phosphorylated. Phosphorylation at Ser-71 and Ser-73 by CDK14 is enhanced during the G2 and M cell cycle phases, and creates a phosphodegron triggering SCF-dependent ubiquitination. -
细胞定位
Nucleus and Cell membrane. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human Cyclin Y protein (Tagged) (ab174060)
SDS-PAGE showing ab174060
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab174060 尚未被引用在任何文献中。