重组人Cyclin E1蛋白(ab119719)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Suitable for: WB, SDS-PAGE
描述
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产品名称
重组人Cyclin E1蛋白 -
纯度
> 90 % SDS-PAGE.
Purity tested by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
73 kDa including tags -
氨基酸
1 to 410
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技术指标
Our Abpromise guarantee covers the use of ab119719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.003% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
常规信息
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别名
- CCNE
- Ccne1
- CCNE1_HUMAN
see all -
功能
Essential for the control of the cell cycle at the G1/S (start) transition. -
组织特异性
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. -
序列相似性
Belongs to the cyclin family. Cyclin E subfamily. -
翻译后修饰
Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human Cyclin E1 protein (ab119719)SDS Page analysis of ab119719
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab119719 被引用在 1 文献中.
- Cai Z et al. LncRNA EILA promotes CDK4/6 inhibitor resistance in breast cancer by stabilizing cyclin E1 protein. Sci Adv 9:eadi3821 (2023). PubMed: 37801505