重组人Cyclin D3/CCND3 + Cdk4蛋白(Active) (ab271459)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 73% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus, GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人Cyclin D3/CCND3 + Cdk4蛋白(Active) -
生物活性
Specific activity = 10 pmole/min/ug.
Assay was done in a Kinase buffer using Rb (0.02 mg/ml) as a substrate with 10 μM ATP at 30°C for 60 min.
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纯度
>= 73 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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氨基酸序列 1
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种属
Human -
序列
MATSRYEPVA EIGVGAYGTV YKARDPHSGH FVALKSVRVP NGGGGGGGLP ISTVREVALL RRLEAFEHPN VVRLMDVCAT SRTDREIKVT LVFEHVDQDL RTYLDKAPPP GLPAETIKDL MRQFLRGLDF LHANCIVHRD LKPENILVTS GGTVKLADFG LARIYSYQMA LTPVVVTLWY RAPEVLLQST YATPVDMWSV GCIFAEMFRR KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR DVSLPRGAFP PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYLHKDEG NPE -
预测分子量
64 kDa including tags -
氨基酸
1 to 303 -
标签
His tag N-Terminus , GST tag N-Terminus -
额外的序列信息
Genbank: NM_000075
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氨基酸序列 2
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种属
Human -
序列
ELLCCEGTRH APRAGPDPRL LGDQRVLQSL LRLEERYVPR ASYFQCVQRE IKPHMRKMLA YWMLEVCEEQ RCEEEVFPLA MNYLDRYLSC VPTRKAQLQL LGAVCMLLAS KLRETTPLTI EKLCIYTDHA VSPRQLRDWE VLVLGKLKWD LAAVIAHDFL AFILHRLSLP RDRQALVKKH AQTFLALCAT DYTFAMYPPS MIATGSIGAA VQGLGACSMS GDELTELLAG ITGTEVDCLR ACQEQIEAAL RESLREASQT SSSPAPKAPR GSSSQGPSQT STPTDVTAIH L -
氨基酸
2 to 292 -
标签
GST tag N-Terminus -
额外的序列信息
Genbank: NM_001760
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技术指标
Our Abpromise guarantee covers the use of ab271459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
Complex of recombinant human CDK4 and Cyclin D3.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.5
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.06% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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细胞定位
Cyclin D3/CCND3: Nucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. Cdk4: Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
图片
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Functional Studies - Recombinant Human CDK4 + CCND3 protein (Tagged) (ab271459)
Functional analysis of ab271459.
Specific activity = 10 pmole/min/ug.
Assay was done in a Kinase buffer using Rb (0.02 mg/ml) as a substrate with 10 μM ATP at 30°C for 60 min.
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SDS-PAGE - Recombinant Human CDK4 + CCND3 protein (Tagged) (ab271459)SDS-PAGE analysis of ab271459.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271459 尚未被引用在任何文献中。