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AB158041

重组人 Cyclin D3/CCND3 蛋白 (GST tag N-Terminus)

Recombinant Human Cyclin D3/CCND3 protein (GST tag N-Terminus)

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Recombinant Human Cyclin D3/CCND3 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 292 aa range, expressed in Wheat germ, suitable for ELISA, WB.

查看别名

G1/S-specific cyclin-D3, CCND3

1 Images
SDS-PAGE - Recombinant Human Cyclin D3/CCND3 protein (GST tag N-Terminus) (AB158041)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Cyclin D3/CCND3 protein (GST tag N-Terminus) (AB158041)

ab158041 on a 12.5% SDS-PAGE stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

ELISA, WB

applications

生物活性

No

访问

P30281

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB116027

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB116027)

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  • 2
Proteins & Peptides

AB56270

Recombinant Human Rb protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Rb protein (AB56270)

  • 0
  • 0
Proteins & Peptides

AB198676

Recombinant Human Histone H1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Histone H1 protein (His tag N-Terminus) (AB198676)

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  • 0
Primary Antibodies

AB134914

Anti-Histone H1.0 antibody [EPR6536]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

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  • 1
Primary Antibodies

AB183338

Anti-Cyclin D3/CCND3 antibody [SP207]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D3/CCND3 antibody [SP207] (AB183338)

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  • 1
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

This product was previously labelled as Cyclin D3.
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序列信息

[{"linker":null,"sequence":"MELLCCEGTRHAPRAGPDPRLLGDQRVLQSLLRLEERYVPRASYFQCVQREIKPHMRKMLAYWMLEVCEEQRCEEEVFPLAMNYLDRYLSCVPTRKAQLQLLGAVCMLLASKLRETTPLTIEKLCIYTDHAVSPRQLRDWEVLVLGKLKWDLAAVIAHDFLAFILHRLSLPRDRQALVKKHAQTFLALCATDYTFAMYPPSMIATGSIGAAVQGLGACSMSGDELTELLAGITGTEVDCLRACQEQIEAALRESLREASQTSSSPAPKAPRGSSSQGPSQTSTPTDVTAIHL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":292,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P30281","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
纯化工艺
Proprietary technique
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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常规信息

功能

Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed : 8114739). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed : 8114739). Hypophosphorylates RB1 in early G(1) phase (PubMed : 8114739). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed : 8114739). Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed : 16782892). Shows transcriptional coactivator activity with ATF5 independently of CDK4 (PubMed : 15358120).

序列相似性

Belongs to the cyclin family. Cyclin D subfamily.

翻译后修饰

Phosphorylation at Thr-283 by MAP kinases is required for ubiquitination and degradation by the DCX(AMBRA1) complex.. Ubiquitinated by the DCX(AMBRA1) complex during the transition from G1 to S cell phase, leading to its degradation: ubiquitination is dependent on Thr-283 phosphorylation (PubMed:33854235, PubMed:33854239). The DCX(AMBRA1) complex represents the major regulator of CCND3 stability during the G1/S transition (PubMed:33854235, PubMed:33854239). Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation (PubMed:22020328).

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed : 8114739). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed : 8114739). Hypophosphorylates RB1 in early G(1) phase (PubMed : 8114739). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed : 8114739). Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed : 16782892). Shows transcriptional coactivator activity with ATF5 independently of CDK4 (PubMed : 15358120).
See full target information G1/S-specific cyclin-D3
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