重组人Cyclin D1 + Cdk4 (mutated T172A，43G-47G to EE)蛋白(Tagged) (ab271457)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人Cyclin D1 + Cdk4 (mutated T172A，43G-47G to EE)蛋白(Tagged) -
纯度
>= 90 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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氨基酸序列 1
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种属
Human -
预测分子量
34 kDa including tags -
氨基酸
1 to 303 -
修饰
mutated T172A -
标签
His tag C-Terminus -
额外的序列信息
Genbank: NM_000075 Residues 43G - 47G replaced with EE
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氨基酸序列 2
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种属
Human -
序列
AYPDANLLND RVLRAMLKAE ETCAPSVSYF KCVQKEVLPS MRKIVATWML EVCEEQKCEE EVFPLAMNYL DRFLSLEPVK KSRLQLLGAT CMFVASKMKE TIPLTAEKLC IYTDNSIRPE ELLQMELLLV NKLKWNLAAM TPHDFIEHFL SKMPEAEENK QIIRKHAQTF VALCATDVKF ISNPPSMVAA GSVVAAVQGL NLRSPNNFLS YYRLTRFLSR VIKCDPDCLR ACQEQIEALL ESSLRQAQQN MDPKAA -
预测分子量
29 kDa -
氨基酸
16 to 271 -
额外的序列信息
Genbank: NM_053056
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描述
重组人Cyclin D1 + Cdk4 (mutated T172A)蛋白(Tagged)
技术指标
Our Abpromise guarantee covers the use of ab271457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
补充说明
Complex of recombinant, full length human CDK4 (mutated T172 A, 43G-47G to EE) and human Cyclin D1.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% Tween, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
常规信息
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细胞定位
Cyclin D1: Nucleus. Cdk4: Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.
图片
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SDS-PAGE - Recombinant Human Cyclin D1 + Cdk4 protein (Tagged) (ab271457)SDS-PAGE analysis of ab271457.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271457 尚未被引用在任何文献中。