重组人CUL-2 + Elongin-B + Elongin-C + RBX1 + VHL蛋白(Tagged) (ab271787)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag N-Terminus, DDDDK tag N-Terminus, DDDDK tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人CUL-2 + Elongin-B + Elongin-C + RBX1 + VHL蛋白(Tagged) -
生物活性
Assay Conditions: ARV-771-mediated binding of VHL to BET bromodomain was assessed using A-screen technology. Various amounts of VHL were incubated in 10-ul reaction with ARV-771 and GSTBRD3 (BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.
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纯度
>= 80 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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氨基酸序列 1
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种属
Human -
序列
AAAMDVDTPS GTNSGAGKKR FEVKKWNAVA LWAWDIVVDN CAICRNHIMD LCIECQANQA SATSEECTVA WGVCNHAFHF HCISRWLKTR QVCPLDNREW EFQKYGH -
预测分子量
13 kDa including tags -
氨基酸
2 to 108 -
标签
His tag N-Terminus
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氨基酸序列 2
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种属
Human -
序列
PRRAENWDEA EVGAEEAGVE EYGPEEDGGE ESGAEESGPE ESGPEELGAE EEMEAGRPRP VLRSVNSREP SQVIFCNRSP RVVLPVWLNF DGEPQPYPTL PPGTGRRIHS YRGHLWLFRD AGTHDGLLVN QTELFVPSLN VDGQPIFANI TLPVYTLKER CLQVVRSLVK PENYRRLDIV RSLYEDLEDH PNVQKDLERL TQERIAHQRM GD -
预测分子量
25 kDa including tags -
氨基酸
2 to 212 -
标签
DDDDK tag N-Terminus
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氨基酸序列 3
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种属
Human -
序列
SLKPRVVDFD ETWNKLLTTI KAVVMLEYVE RATWNDRFSD IYALCVAYPE PLGERLYTET KIFLENHVRH LHKRVLESEE QVLVMYHRYW EEYSKGADYM DCLYRYLNTQ FIKKNKLTEA DLQYGYGGVD MNEPLMEIGE LALDMWRKLM VEPLQAILIR MLLREIKNDR GGEDPNQKVI HGVINSFVHV EQYKKKFPLK FYQEIFESPF LTETGEYYKQ EASNLLQESN CSQYMEKVLG RLKDEEIRCR KYLHPSSYTK VIHECQQRMV ADHLQFLHAE CHNIIRQEKK NDMANMYVLL RAVSTGLPHM IQELQNHIHD EGLRATSNLT QENMPTLFVE SVLEVHGKFV QLINTVLNGD QHFMSALDKA LTSVVNYREP KSVCKAPELL AKYCDNLLKK SAKGMTENEV EDRLTSFITV FKYIDDKDVF QKFYARMLAK RLIHGLSMSM DSEEAMINKL KQACGYEFTS KLHRMYTDMS VSADLNNKFN NFIKNQDTVI DLGISFQIYV LQAGAWPLTQ APSSTFAIPQ ELEKSVQMFE LFYSQHFSGR KLTWLHYLCT GEVKMNYLGK PYVAMVTTYQ MAVLLAFNNS ETVSYKELQD STQMNEKELT KTIKSLLDVK MINHDSEKED IDAESSFSLN MNFSSKRTKF KITTSMQKDT PQEMEQTRSA VDEDRKMYLQ AAIVRIMKAR KVLRHNALIQ EVISQSRARF NPSISMIKKC IEVLIDKQYI ERSQASADEY SYVA -
预测分子量
88 kDa including tags -
氨基酸
2 to 745 -
标签
DDDDK tag N-Terminus
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氨基酸序列 4
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种属
Human -
序列
DVFLMIRRHK TTIFTDAKES STVFELKRIV EGILKRPPDE QRLYKDDQLL DDGKTLGECG FTSQTARPQA PATVGLAFRA DDTFEALCIE PFSSPPELPD VMKPQDSGSS ANEQAVQ -
预测分子量
14 kDa including tags -
氨基酸
2 to 118 -
标签
DDDDK tag N-Terminus
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氨基酸序列 5
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种属
Human -
序列
DGEEKTYGGC EGPDAMYVKL ISSDGHEFIV KREHALTSGT IKAMLSGPGQ FAENETNEVN FREIPSHVLS KVCMYFTYKV RYTNSSTEIP EFPIAPEIAL ELLMAANFLD C -
预测分子量
13 kDa including tags -
氨基酸
2 to 112 -
标签
His tag N-Terminus
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描述
重组人Cullin 2/CUL-2 + RBX1 + Von Hippel Lindau/VHL + TCEB2/Elongin-B + Elongin-C蛋白(Tagged)
技术指标
Our Abpromise guarantee covers the use of ab271787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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细胞定位
RBX1: Cytoplasm. Nucleus. Von Hippel Lindau/VHL: Cytoplasm. Membrane. Nucleus. Found predominantly in the cytoplasm and with less amounts nuclear or membrane-associated and Cytoplasm. Nucleus. Equally distributed between the nucleus and the cytoplasm but not membrane-associated. TCEB2/Elongin-B: Nucleus. Elongin-C: Nucleus.
图片
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SDS-PAGE - Recombinant Human Cullin 2/CUL-2 + RBX1 + Von Hippel Lindau/VHL + TCEB2/Elongin-B protein (Tagged) (ab271787)
SDS-PAGE analysis of ab271787.
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Functional Studies - Recombinant Human Cullin 2/CUL-2 + RBX1 + Von Hippel Lindau/VHL + TCEB2/Elongin-B protein (Tagged) (ab271787)Functional studies of ab271787.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab271787 被引用在 1 文献中.
- Wang L et al. SARS-CoV-2 ORF10 impairs cilia by enhancing CUL2ZYG11B activity. J Cell Biol 221:N/A (2022). PubMed: 35674692