重组人CSK蛋白(ab60764)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人CSK蛋白
参阅全部 CSK 蛋白酶 -
纯度
> 85 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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相关产品
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Related Products
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- C SRC
- C SRC kinase
- C src Tyrosine Kinase
see all -
功能
Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases. -
组织特异性
Expressed in lung and macrophages. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻译后修饰
Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro. -
细胞定位
Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts. - Information by UniProt
图片
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Functional Studies - Recombinant human CSK protein (ab60764)Sample Kinase Activity Plot.
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SDS-PAGE - Recombinant human CSK protein (ab60764)ab60764 on SDS-PAGE, MW ~52kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab60764 尚未被引用在任何文献中。