AB84062

重组人Cripto1/CRIPTO蛋白(Fc Chimera)

Recombinant human Cripto1/CRIPTO protein (Fc Chimera)

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Recombinant human Cripto1/CRIPTO protein (Fc Chimera) is a Human Fragment protein, in the 31 to 169 aa range, expressed in HEK 293 cells, with >95%, suitable for FuncS, SDS-PAGE.

查看别名

CRIPTO-1, TDGF1, CRIPTO, Protein Cripto, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1, CRGF

3 Images

Functional Studies - Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (AB84062)

FuncS

Unknown

Functional Studies - Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (AB84062)

Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.

The triangle indicates theoretical pI and MW of the protein.

SDS-PAGE - Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (AB84062)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (AB84062)

A sample of ab84062 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4 20% Tris-HCl 2D gel. Approximately 40 μg of protein was loaded; Gel was stained using Deep Purple™. The spot train indicates the presence of multiple glycoforms. Spots within the spot train were cut from the gel and identified as Cripto1/CRIPTO (Fc Chimera) by protein mass fingerprinting.

SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (AB84062)

Lane 1 – MW markers; Lane 2 – ab84062; Lane 3 – ab84062 treated with PNGase F to remove potential N linked glycans; Lane 4 – ab84062 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.

Drop in MW after treatment with PNGase F indicates the presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.

关键信息

纯度

>95% SDS-PAGE

表达系统

HEK 293 cells

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

200 ng/ml ab84062 induces ERK1 and ERK2 phosphorylation in human umbilical vein endothelial (HUVEC) cells

访问

P13385

不含动物源

不含载体蛋白

种属

Human

复溶

Reconstitute in 0.5 mL of PBS

存储溶液

Constituents: 10% Trehalose, 1% Human serum albumin

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Under reducing conditions ab84062 migrates as a broad band between 45 and 55 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified Cripto-1 - Fc Chimera that has a predicted monomeric molecular mass of 42.8 kDa." } } }

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产品详情

200 ng/ml of this Chimera induces ERK1 and ERK2 phosphorylation in human umbilical vein endothelial (HUVEC) cells. This product was previously labelled as Cripto1

This product was previously labelled as Cripto1

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序列信息

[{"sequence":"Theoretical sequence:\r\nLGHQEFARPSRGYLAFRDDSIWPQEEPAIRPRSSQRVPPMGIQHSKEL\r\nNRTCCLNGGTCMLGSFCACPPSFYGRNCEHDVRKENCGSVPHDTWLPK\r\nKCSLCKCWHGQLRCFPQAFLPGCDGLVMDEHLVASRTPELPPSGSSNT\r\nKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTP\r\nEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSV\r\nLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPP\r\nSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSD\r\nGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":169,"aminoAcidStart":31,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P13385","tags":[]}]

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性能和储存信息

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle|Reconstitute for long term storage

True

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特殊说明

形式

Lyophilized

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常规信息

功能

GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells (PubMed : 27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed : 11909953).

序列相似性

Belongs to the EGF-CFC (Cripto-1/FRL1/Cryptic) family.

翻译后修饰

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, it is processed by GPI processing phospholipase A2 (TMEM8A), removing an acyl-chain at the sn-2 position of GPI and releasing CRIPTO as a lysophosphatidylinositol-bearing form, which is further cleaved by phospholipase D (GPLD1) into a soluble form.

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information CRIPTO

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重组人Cripto1/CRIPTO蛋白(Fc Chimera)

Recombinant human Cripto1/CRIPTO protein (Fc Chimera)

Functional Studies - Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (AB84062)

SDS-PAGE - Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (AB84062)

SDS-PAGE - Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (AB84062)

关键信息

纯度

表达系统

标签

应用

生物活性

生物学活性

访问

不含动物源

不含载体蛋白

种属

复溶

存储溶液