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AB162449

重组人 CRBN 蛋白 (GST tag N-Terminus)

Recombinant Human CRBN protein (GST tag N-Terminus)

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Recombinant Human CRBN protein is a Human Full Length protein, in the 1 to 442 aa range, expressed in Wheat germ and suitable for western blot, SDS-PAGE and ELISA. The predicted molecular weight of ab162449 protein is 76.9 kDa.

- Save time and ensure accurate results - use our Cereblon (CRBN) protein as a control

查看别名

AD-006, CRBN, Protein cereblon

1 Images
SDS-PAGE - Recombinant Human CRBN protein (GST tag N-Terminus) (AB162449)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human CRBN protein (GST tag N-Terminus) (AB162449)

ab162449 on a 12.5% SDS-PAGE stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

ELISA, SDS-PAGE, WB

applications

生物活性

No

访问

Q96SW2

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB325996

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 12 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 12 bands) (AB325996)

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  • 0
Proteins & Peptides

AB271467

Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (DDDDK tag N-Terminus, His tag N-Terminus)

Functional Studies - Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (DDDDK tag N-Terminus, His tag N-Terminus) (AB271467)

  • 0
  • 0
Proteins & Peptides

AB289731

Recombinant Human VHL Protein

HPLC - Recombinant Human VHL Protein (AB289731)

  • 0
  • 0
Primary Antibodies

AB244223

Anti-CRBN antibody [4D6]

BOND RX™ Validated|KO Validated

Western blot - Anti-CRBN antibody [4D6] (AB244223)

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  • 1
Proteins & Peptides

AB125625

Recombinant Human STAT6 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human STAT6 protein (AB125625)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Ensure the validity of your result using our recombinant human Cereblon (CRBN) protein ab162449 as a positive control in western blot and SDS-PAGE.

Analyze your CRBN ELISA data using the ab162449 protein to generate and plot a standard curve.


Check out our protein gel staining guide for SDS-PAGE here

Check out of western blot protocol for more information here

Check out our ELISA protocol for more information here.
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序列信息

[{"linker":null,"sequence":"MAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL","proteinLength":"Full Length","predictedMolecularWeight":"76.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":442,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q96SW2","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
纯化工艺
Proprietary technique
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CRBN also known as cereblon is a protein involved in the regulation of protein homeostasis. It has a molecular weight of approximately 51 kDa. CRBN is expressed in various tissues including the brain kidney and muscle. It functions as a substrate receptor for the CRL4-CRBN E3 ubiquitin ligase complex recognizing proteins for ubiquitination and degradation. The chemical structure of thalidomide shows binding affinity to CRBN influencing its function.
Biological function summary

Cereblon plays a role in the regulation of protein stability and degradation. It forms part of the CRL4-CRBN complex which tags specific proteins for ubiquitination and subsequent proteasomal degradation. This function is critical for maintaining cellular protein balance. Additionally CRBN modulates immune response and has immunomodulator properties influencing cell-mediated responses.

Pathways

CRBN participates in the ubiquitin-proteasome pathway which regulates protein turnover and quality control within cells. Moreover CRBN is involved in immune signaling pathways partly by modulating the expression of various cytokines and immune-related proteins. It interacts with proteins such as DDB1 and CUL4 within these pathways impacting cellular responses to stress and immune challenges.

CRBN is associated with multiple myeloma and intellectual disability. Cereblon acts as a target for drugs like thalidomide and lenalidomide which are used in the treatment of multiple myeloma by altering ubiquitination processes. In intellectual disability alterations in CRBN function or expression can disrupt neuronal development and signaling. CRBN interacts with proteins like IKZF1 and IKZF3 within these contexts affecting their roles in disease progression and treatment responses.
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常规信息

功能

Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2, ILF2 or GLUL (PubMed : 26990986, PubMed : 33009960). Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8 (PubMed : 20223979, PubMed : 24328678, PubMed : 25043012, PubMed : 25108355). Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons (PubMed : 18414909, PubMed : 29530986). Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1 (PubMed : 18414909). May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism (By similarity). Plays a negative role in TLR4 signaling by interacting with TRAF6 and ECSIT, leading to inhibition of ECSIT ubiquitination, an important step of the signaling (PubMed : 31620128).

序列相似性

Belongs to the CRBN family.

翻译后修饰

Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex.

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产品实验方案

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靶点信息

Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2, ILF2 or GLUL (PubMed : 26990986, PubMed : 33009960). Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8 (PubMed : 20223979, PubMed : 24328678, PubMed : 25043012, PubMed : 25108355). Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons (PubMed : 18414909, PubMed : 29530986). Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1 (PubMed : 18414909). May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism (By similarity). Plays a negative role in TLR4 signaling by interacting with TRAF6 and ECSIT, leading to inhibition of ECSIT ubiquitination, an important step of the signaling (PubMed : 31620128).
See full target information CRBN
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