重组人CRBN + DDB1 + CUL-4A + RBX1蛋白(Tagged) (ab271467)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus, DDDDK tag N-Terminus, His tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
-
产品名称
重组人CRBN + DDB1 + CUL-4A + RBX1蛋白(Tagged) -
生物活性
Cereblon-BRD3 interaction was assessed using A-screen technology. Various amounts of ab271467 were incubated in 10-ul reaction with dBET1 and GST-BRD3(BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.
-
纯度
>= 90 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
-
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
-
氨基酸序列 1
-
种属
Human -
序列
AGEGDQQDAA HNMGNHLPLL PAESEEEDEM EVEDQDSKEA KKPNIINFDT SLPTSHTYLG ADMEEFHGRT LHDDDSCQVI PVLPQVMMIL IPGQTLPLQL FHPQEVSMVR NLIQKDRTFA VLAYSNVQER EAQFGTTAEI YAYREEQDFG IEIVKVKAIG RQRFKVLELR TQSDGIQQAK VQILPECVLP STMSAVQLES LNKCQIFPSK PVSREDQCSY KWWQKYQKRK FHCANLTSWP RWLYSLYDAE TLMDRIKKQL REWDENLKDD SLPSNPIDFS YRVAACLPID DVLRIQLLKI GSAIQRLRCE LDIMNKCTSL CCKQCQETEI TTKNEIFSLS LCGPMAAYVN PHGYVHETLT VYKACNLNLI GRPSTEHSWF PGYAWTVAQC KICASHIGWK FTATKKDMSP QKFWGLTRSA LLPTIPDTED EISPDKVILC L -
预测分子量
51 kDa including tags -
氨基酸
2 to 442 -
标签
DDDDK tag N-Terminus -
额外的序列信息
GenBank: NM_016302
-
氨基酸序列 2
-
种属
Human -
序列
MSYNYVVTAQ KPTAVNGCVT GHFTSAEDLN LLIAKNTRLE IYVVTAEGLR PVKEVGMYGK IAVMELFRPK GESKDLLFIL TAKYNACILE YKQSGESIDI ITRAHGNVQD RIGRPSETGI IGIIDPECRM IGLRLYDGLF KVIPLDRDNK ELKAFNIRLE ELHVIDVKFL YGCQAPTICF VYQDPQGRHV KTYEVSLREK EFNKGPWKQE NVEAEASMVI AVPEPFGGAI IIGQESITYH NGDKYLAIAP PIIKQSTIVC HNRVDPNGSR YLLGDMEGRL FMLLLEKEEQ MDGTVTLKDL RVELLGETSI AECLTYLDNG VVFVGSRLGD SQLVKLNVDS NEQGSYVVAM ETFTNLGPIV DMCVVDLERQ GQGQLVTCSG AFKEGSLRII RNGIGIHEHA SIDLPGIKGL WPLRSDPNRE TDDTLVLSFV GQTRVLMLNG EEVEETELMG FVDDQQTFFC GNVAHQQLIQ ITSASVRLVS QEPKALVSEW KEPQAKNISV ASCNSSQVVV AVGRALYYLQ IHPQELRQIS HTEMEHEVAC LDITPLGDSN GLSPLCAIGL WTDISARILK LPSFELLHKE MLGGEIIPRS ILMTTFESSH YLLCALGDGA LFYFGLNIET GLLSDRKKVT LGTQPTVLRT FRSLSTTNVF ACSDRPTVIY SSNHKLVFSN VNLKEVNYMC PLNSDGYPDS LALANNSTLT IGTIDEIQKL HIRTVPLYES PRKICYQEVS QCFGVLSSRI EVQDTSGGTT ALRPSASTQA LSSSVSSSKL FSSSTAPHET SFGEEVEVHN LLIIDQHTFE VLHAHQFLQN EYALSLVSCK LGKDPNTYFI VGTAMVYPEE AEPKQGRIVV FQYSDGKLQT VAEKEVKGAV YSMVEFNGKL LASINSTVRL YEWTTEKELR TECNHYNNIM ALYLKTKGDF ILVGDLMRSV LLLAYKPMEG NFEEIARDFN PNWMSAVEIL DDDNFLGAEN AFNLFVCQKD SAATTDEERQ HLQEVGLFHL GEFVNVFCHG SLVMQNLGET STPTQGSVLF GTVNGMIGLV TSLSESWYNL LLDMQNRLNK VIKSVGKIEH SFWRSFHTER KTEPATGFID GDLIESFLDI SRPKMQEVVA NLQYDDGSGM KREATADDLI KVVEELTRIH -
预测分子量
128 kDa including tags -
氨基酸
1 to 1140 -
标签
DDDDK tag N-Terminus -
额外的序列信息
GenBank: NM_001923
-
氨基酸序列 3
-
种属
Human -
序列
ADEAPRKGSF SALVGRTNGL TKPAALAAAP AKPGGAGGSK KLVIKNFRDR PRLPDNYTQD TWRKLHEAVR AVQSSTSIRY NLEELYQAVE NLCSHKVSPM LYKQLRQACE DHVQAQILPF REDSLDSVLF LKKINTCWQD HCRQMIMIRS IFLFLDRTYV LQNSTLPSIW DMGLELFRTH IISDKMVQSK TIDGILLLIE RERSGEAVDR SLLRSLLGML SDLQVYKDSF ELKFLEETNC LYAAEGQRLM QEREVPEYLN HVSKRLEEEG DRVITYLDHS TQKPLIACVE KQLLGEHLTA ILQKGLDHLL DENRVPDLAQ MYQLFSRVRG GQQALLQHWS EYIKTFGTAI VINPEKDKDM VQDLLDFKDK VDHVIEVCFQ KNERFVNLMK ESFETFINKR PNKPAELIAK HVDSKLRAGN KEATDEELER TLDKIMILFR FIHGKDVFEA FYKKDLAKRL LVGKSASVDA EKSMLSKLKH ECGAAFTSKL EGMFKDMELS KDIMVHFKQH MQNQSDSGPI DLTVNILTMG YWPTYTPMEV HLTPEMIKLQ EVFKAFYLGK HSGRKLQWQT TLGHAVLKAE FKEGKKEFQV SLFQTLVLLM FNEGDGFSFE EIKMATGIED SELRRTLQSL ACGKARVLIK SPKGKEVEDG DKFIFNGEFK HKLFRIKINQ IQMKETVEEQ VSTTERVFQD RQYQIDAAIV RIMKMRKTLG HNLLVSELYN QLKFPVKPGD LKKRIESLID RDYMERDKDN PNQYHYVA -
预测分子量
88 kDa including tags -
氨基酸
2 to 729 -
标签
His tag N-Terminus -
额外的序列信息
Gen Bank: NM_003589
-
氨基酸序列 4
-
种属
Human -
序列
AAAMDVDTPS GTNSGAGKKR FEVKKWNAVA LWAWDIVVDN CAICRNHIMD LCIECQANQA SATSEECTVA WGVCNHAFHF HCISRWLKTR QVCPLDNREW EFQKYGH -
氨基酸
2 to 108 -
标签
His tag N-Terminus -
额外的序列信息
GenBank: NM_014248
-
-
描述
重组人DDB1 + Cullin 4A/CUL-4A + RBX1 + CRBN蛋白(Active)
技术指标
Our Abpromise guarantee covers the use of ab271467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
SDS-PAGE
Functional Studies
-
形式
Liquid -
补充说明
Co-expressed in HEK-293 cells.
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
细胞定位
DDB1: Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage. RBX1: Cytoplasm. Nucleus. CRBN: Cytoplasm. Nucleus. Membrane.
图片
-
Functional Studies - Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged) (ab271467)
Cereblon-BRD3 interaction.
Various amounts of ab271467 were incubated in 10-ul reaction with dBET1 and GST-BRD3(BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.
-
SDS-PAGE - Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged) (ab271467)SDS-PAGE analysis of ab271467.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab271467 尚未被引用在任何文献中。