重组人冠状病毒SARS-CoV-2 Spike Glycoprotein S1 (Active) (ab273068)
Key features and details
- Expression system: CHO cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Indirect ELISA, SDS-PAGE, Functional Studies
描述
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产品名称
重组人冠状病毒SARS-CoV-2 Spike Glycoprotein S1 (Active)
参阅全部 SARS-CoV-2 Spike Glycoprotein S1 蛋白酶 -
生物活性
Flow cytometry assay showing ab273068 can bind to ACE2 overexpressing cells. ACE2 overexpressing cells were stained with ab273068, followed by an anti-spike protein antibody, and a fluorescence-conjugated secondary antibody.
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纯度
> 90 % SDS-PAGE.
Purity is lot specific. Please contact our technical Support team for details. -
表达系统
CHO cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human coronavirus -
序列
VNLTTRTQLP PAYTNSFTRG VYYPDKVFRS SVLHSTQDLF LPFFSNVTWF HAIHVSGTNG TKRFDNPVLP FNDGVYFAST EKSNIIRGWI FGTTLDSKTQ SLLIVNNATN VVIKVCEFQF CNDPFLGVYY HKNNKSWMES EFRVYSSANN CTFEYVSQPF LMDLEGKQGN FKNLREFVFK NIDGYFKIYS KHTPINLVRD LPQGFSALEP LVDLPIGINI TRFQTLLALH RSYLTPGDSS SGWTAGAAAY YVGYLQPRTF LLKYNENGTI TDAVDCALDP LSETKCTLKS FTVEKGIYQT SNFRVQPTES IVRFPNITNL CPFGEVFNAT RFASVYAWNR KRISNCVADY SVLYNSASFS TFKCYGVSPT KLNDLCFTNV YADSFVIRGD EVRQIAPGQT GKIADYNYKL PDDFTGCVIA WNSNNLDSKV GGNYNYLYRL FRKSNLKPFE RDISTEIYQA GSTPCNGVEG FNCYFPLQSY GFQPTNGVGY QPYRVVVLSF ELLHAPATVC GPKKSTNLVK NKCVNFNFNG LTGTGVLTES NKKFLPFQQF GRDIADTTDA VRDPQTLEIL DITPCSFGGV SVITPGTNTS NQVAVLYQDV NCTEVPVAIH ADQLTPTWRV YSTGSNVFQT RAGCLIGAEH VNNSYECDIP IGAGICASYQ TQTNSPRRAR -
预测分子量
76 kDa -
实际分子量
125 kDa -
氨基酸
16 to 685 -
标签
His tag C-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab273068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Indirect ELISA
SDS-PAGE
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Preservative: 1.02% Imidazole
Constituents: 0.82% Sodium phosphate, 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
图片
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Indirect ELISA - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (ab273068)
Indirect ELISA showing primary antibody ab273073 (CR3022, human chimeric) binding to the antigens ab272105 (recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (sheep Fc fusion)) and ab273068 (recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active)). Plates were coated with 100ng/well ab272105 or ab273068 and binding of ab273073 assessed in serial dilution from 200ng/ml primary antibody in duplicate. Binding was detected using ab98624, an anti-human Fc secondary conjugated to HRP. Data are represented as the mean and error bars represent standard deviation.
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Indirect ELISA - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (ab273068)Indirect ELISA showing primary antibody ab273074 (CR3022, rabbit chimeric) binding to the antigen ab273068 (recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active)). Plates were coated with 100ng/well ab272105 or ab273068 and binding of ab273073 assessed in serial dilution from 200ng/ml primary antibody in duplicate. Binding was detected using ab97080, an anti-rabbit H&L secondary conjugated to HRP. Data are represented as the mean and error bars represent standard deviation.
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Functional Studies - Recombinant SARS Coronavirus Spike Glycoprotein Mosaic (S1) (Active) (ab273068)Flow cytometry assay showing ab273068 can bind to ACE2 overexpressing cells. ACE2 overexpressing cells were stained with ab273068, followed by an anti-spike protein antibody, and a fluorescence-conjugated secondary antibody.
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SDS-PAGE - Recombinant SARS Coronavirus Spike Glycoprotein Mosaic (S1) (Active) (ab273068)SDS-PAGE analysis of ab273068.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab273068 被引用在 16 文献中.
- Prieto-Villalobos J et al. SARS-CoV-2 spike protein S1 activates Cx43 hemichannels and disturbs intracellular Ca2+ dynamics. Biol Res 56:56 (2023). PubMed: 37876016
- Wallace S et al. Multiplexed Biosensing of Proteins and Virions with Disposable Plasmonic Assays. ACS Sens 8:3338-3348 (2023). PubMed: 37610841
- Kim JS et al. Direct activation of airway sensory C-fibers by SARS-CoV-2 S1 spike protein. Physiol Rep 11:e15900 (2023). PubMed: 38123162
- Chittasupho C et al. Targeting Spike Glycoprotein S1 Mediated by NLRP3 Inflammasome Machinery and the Cytokine Releases in A549 Lung Epithelial Cells by Nanocurcumin. Pharmaceuticals (Basel) 16:N/A (2023). PubMed: 37375809
- Brazaca LC et al. Electrochemical immunosensors using electrodeposited gold nanostructures for detecting the S proteins from SARS-CoV and SARS-CoV-2. Anal Bioanal Chem 414:5507-5517 (2022). PubMed: 35169906