重组人冠状病毒SARS-CoV-2 Spike Glycoprotein RBD (Active) (ab273065)
Key features and details
- Expression system: CHO cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: ELISA, Flow Cyt, SDS-PAGE
描述
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产品名称
重组人冠状病毒SARS-CoV-2 Spike Glycoprotein RBD (Active)
参阅全部 SARS-CoV-2 Spike Glycoprotein RBD 蛋白酶 -
生物活性
Binding ability measured in a functional ELISA. Immobilized Human ACE2 protein at 2 ug/ml (100 ul/well) can bind to ab273065.
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纯度
> 90 % SDS-PAGE.
Purity is lot specific. Please contact our technical Support team for details. -
表达系统
CHO cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human coronavirus -
序列
RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVL YNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKI ADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDI STEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELL HAPATVCGPKKSTNLVKNKCVNFGSGGGSGGHHHHHH -
实际分子量
39 kDa including tags -
氨基酸
319 to 541 -
标签
His tag C-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab273065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
ELISA
Flow Cytometry
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Preservative: 1.02% Imidazole
Constituents: 0.82% Sodium phosphate, 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
图片
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SDS-PAGE - Recombinant SARS-CoV-2 Spike Glycoprotein RBD (Active) (ab273065)
SDS-PAGE analysis of ab273065.
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Flow Cytometry - Recombinant SARS-CoV-2 Spike Glycoprotein RBD (Active) (ab273065)Flow cytometry assay showing ab273065 can bind to ACE2 overexpressing cells. ACE2 overexpressing cells were labelledwith ab273065, followed by an anti-spike protein antibody, and a fluorescence-conjugated secondary antibody.
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Functional Studies - Recombinant SARS-CoV-2 Spike Glycoprotein RBD (Active) (ab273065)Binding ability measured in a funstional ELISA. Immobilized Human ACE2 protein at 2 ug/ml (100 ul/well) can bind to ab273065.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab273065 被引用在 9 文献中.
- Tsilioni I & Theoharides TC Recombinant SARS-CoV-2 Spike Protein Stimulates Secretion of Chymase, Tryptase, and IL-1β from Human Mast Cells, Augmented by IL-33. Int J Mol Sci 24:N/A (2023). PubMed: 37298438
- Trzaskowski M et al. Portable Surface Plasmon Resonance Detector for COVID-19 Infection. Sensors (Basel) 23:N/A (2023). PubMed: 37112287
- Materón EM et al. Colorimetric Detection of SARS-CoV-2 Using Plasmonic Biosensors and Smartphones. ACS Appl Mater Interfaces 14:54527-54538 (2022). PubMed: 36454041
- Tripathy SP et al. Femtomolar detection of SARS-CoV-2 via peptide beacons integrated on a miniaturized TIRF microscope. Sci Adv 8:eabn2378 (2022). PubMed: 36001655
- Gerasimenko JV et al. SARS-CoV-2 S Protein Subunit 1 Elicits Ca2+ Influx - Dependent Ca2+ Signals in Pancreatic Stellate Cells and Macrophages In Situ. Function (Oxf) 3:zqac002 (2022). PubMed: 35284826
- Abomughaid MM et al. ZnO-chlorogenic acid nanostructured complex inhibits Covid-19 pathogenesis and increases hydroxychloroquine efficacy. J King Saud Univ Sci 34:102296 (2022). PubMed: 36062198
- Li J & Lillehoj PB Microfluidic Magneto Immunosensor for Rapid, High Sensitivity Measurements of SARS-CoV-2 Nucleocapsid Protein in Serum. ACS Sens 6:1270-1278 (2021). PubMed: 33629833
- Nguyen NHL et al. COVID-19 Spike Protein Induced Phononic Modification in Antibody-Coupled Graphene for Viral Detection Application. ACS Nano 15:11743-11752 (2021). PubMed: 34128653
- Tichauer JE et al. Characterization of the Modulatory Effect of Hydroxychloroquine on ACE2 Activity: New Insights in relation to COVID-19. Biomed Res Int 2021:6614000 (2021). PubMed: 34337036