重组人冠状病毒SARS-CoV-2 spike glycoprotein (His tag) (ab281471)
Key features and details
- Expression system: CHO cells
- Purity: > 95% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人冠状病毒SARS-CoV-2 spike glycoprotein (His tag)
参阅全部 SARS-CoV-2 Spike Glycoprotein 蛋白酶 -
纯度
> 95 % SDS-PAGE.
Immobilized metal affinity chromatography -
表达系统
CHO cells -
Accession
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蛋白长度
Full length protein -
无动物成分
Yes -
性质
Recombinant -
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种属
Human coronavirus -
序列
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHS TQDLFLPFFSNVTWFHAIHVSGTNGTKRFD NPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKV CEFQFCNDPFLGVYYHKNNKSWMESEFRVY SSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPI NLVRDLPQGFSALEPLVDLPIGINITRFQT LLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVD CALDPLSETKCTLKSFTVEKGIYQTSNFRV QPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYN SASFSTFKCYGVSPTKLNDLCFTNVYADSF VIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYN YLYRLFRKSNLKPFERDISTEIYQAGSTPC NGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKS TNLVKNKCVNFNFNGLTGTGVLTESNKKFL PFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAV LYQDVNCTEVPVAIHADQLTPTWRVYSTGS NVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPSRASSVASQ SIIAYTMSLGAENSVAYSNNSIAIPTNFTI SVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGI AVEQDKNTQEVFAQVKQIYKTPPIKDFGGF NFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLI CAQKFNGLTVLPPLLTDEMIAQYTSALLAG TITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSA IGKIQDSLSSTASALGKLQDVVNQNAQALN TLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQ QLIRAAEIRASANLAATKMSECVLGQSKRV DFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFP REGVFVSNGTHWFVTQRNFYEPQIITTDNT FVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDIS GINASVVNIQKEIDRLNEVAKNLNESLIDL QELGKYEQYIKGSGGSGGGSHHHHHH -
预测分子量
143 kDa including tags -
实际分子量
170 kDa including tags -
氨基酸
1 to 1211 -
标签
His tag C-Terminus -
额外的序列信息
The furin cleavage site (aa 682-685) is mutated from RRAR to SRAS and the transmembrane domain and intravirion part replaced with a glycine-serine linker. Wuhan-Hu-1 isolate. NCBI: YP_009724390.1
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技术指标
Our Abpromise guarantee covers the use of ab281471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituent: 99% DPBS
图片
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SDS-PAGE - Recombinant Human SARS-CoV-2 spike glycoprotein (His tag) (ab281471)
SDS-PAGE of ab281471.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab281471 被引用在 2 文献中.
- Tsilioni I & Theoharides TC Recombinant SARS-CoV-2 Spike Protein Stimulates Secretion of Chymase, Tryptase, and IL-1β from Human Mast Cells, Augmented by IL-33. Int J Mol Sci 24:N/A (2023). PubMed: 37298438
- Agarwal DK et al. Highly sensitive and ultra-rapid antigen-based detection of SARS-CoV-2 using nanomechanical sensor platform. Biosens Bioelectron 195:113647 (2022). PubMed: 34583103
