重组人CLK2蛋白(ab63190)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人CLK2蛋白 -
纯度
> 80 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
氨基酸
137 to 499
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技术指标
Our Abpromise guarantee covers the use of ab63190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- CDC like kinase 2
- CDC-like kinase 2
- CLK 2
see all -
功能
Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylates on all three types of residues. -
细胞定位
Nucleus. - Information by UniProt
图片
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Functional Studies - Recombinant human CLK2 protein (ab63190)The specific activity of CLK2 (ab63190) was determined to be 60 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human CLK2 protein (ab63190)SDS PAGE analysis of ab63190 -
SDS-PAGE - Recombinant human CLK2 protein (ab63190)SDS PAGE analysis of ab63190 -
Functional Studies - Recombinant human CLK2 protein (ab63190)Sample Kinase Activity Plot.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab63190 被引用在 1 文献中.
- Patel A et al. Induction of beige-like adipocyte markers and functions in 3T3-L1 cells by Clk1 and PKCβII inhibitory molecules. J Cell Mol Med 26:4183-4194 (2022). PubMed: 35801494