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AB160295

重组人CH25H蛋白

Recombinant Human CH25H protein

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Recombinant Human CH25H protein is a Human Full Length protein, in the 1 to 272 aa range, expressed in Wheat germ, suitable for ELISA, WB.

查看别名

Cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, h25OH, CH25H

1 Images
SDS-PAGE - Recombinant Human CH25H protein (AB160295)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CH25H protein (AB160295)

ab160295 on a 12.5% SDS-PAGE stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

WB, ELISA

applications

生物活性

No

访问

O95992

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVGFCLPFVVLDILCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSPALLPHEAPELLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHDLHHSHFNCNFAPYFTHWDKILGTLRTASVPAR","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":272,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O95992","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The CH25H protein also known as cholesterol 25-hydroxylase catalyzes the conversion of cholesterol into 25-hydroxycholesterol. This enzyme has a mass of approximately 33 kDa. It expresses widely in tissues but its level of expression is highest in macrophages and the liver. The protein is part of the enzymatic group that modulates lipid homeostasis and is integral to host defense mechanisms.
Biological function summary

Cholesterol 25-hydroxylase plays a significant role in lipid metabolism and immune regulation. It produces 25-hydroxycholesterol a potent oxysterol which exerts effects on lipid metabolic processes and immune cell signaling. While not part of a large multiprotein complex CH25H's activity impacts cellular cholesterol levels and potentially influences the production of certain signaling molecules.

Pathways

Cholesterol 25-hydroxylase contributes to the regulation of lipid metabolic pathways and innate immune responses. Within the lipid metabolism pathway CH25H interacts with proteins like LXR (liver X receptor) affecting cholesterol homeostasis. The enzyme also influences immune regulation by altering the production of signaling molecules that modulate the interferon response affecting pathways related to inflammation and innate immunity.

Cholesterol 25-hydroxylase has connections to atherosclerosis and viral infections. In the context of atherosclerosis its function in regulating cholesterol levels can influence plaque formation in arteries. The protein's involvement in immune response modulation links it to the body's defense against viral infections. Additionally CH25H's activity interacts with proteins involved in the immune response such as IFN-induced proteins to mount an antiviral state.
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特殊说明

形式

Liquid

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常规信息

功能

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed : 9852097). Plays a key role in cell positioning and movement in lymphoid tissues : 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (By similarity). May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing (PubMed : 9852097). As an interferon-stimulated gene, has broad antiviral activities against a wide range of enveloped viruses, such as vesicular stomatitis virus (VSV) and SARS coronavirus-2 (SARS-CoV-2). Its product, 25-hydroxycholesterol, activates the ER-localized enzyme ACAT to induce internalization of accessible cholesterol on the plasma membrane and restricts SARS-CoV-2 S protein-mediated fusion which inhibits virus replication (PubMed : 32944968, PubMed : 33239446). In testis, production of 25-hydroxycholesterol by macrophages plays a role in Leydig cell differentiation (By similarity). Required to restrain inflammation in macrophages : production of 25-hydroxycholesterol protects macrophages from cholesterol overload, thereby preventing mitochondrial DNA release and subsequent activation of the AIM2 inflammasome (By similarity).

序列相似性

Belongs to the sterol desaturase family.

翻译后修饰

N-glycosylated.

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产品实验方案

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靶点信息

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed : 9852097). Plays a key role in cell positioning and movement in lymphoid tissues : 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (By similarity). May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing (PubMed : 9852097). As an interferon-stimulated gene, has broad antiviral activities against a wide range of enveloped viruses, such as vesicular stomatitis virus (VSV) and SARS coronavirus-2 (SARS-CoV-2). Its product, 25-hydroxycholesterol, activates the ER-localized enzyme ACAT to induce internalization of accessible cholesterol on the plasma membrane and restricts SARS-CoV-2 S protein-mediated fusion which inhibits virus replication (PubMed : 32944968, PubMed : 33239446). In testis, production of 25-hydroxycholesterol by macrophages plays a role in Leydig cell differentiation (By similarity). Required to restrain inflammation in macrophages : production of 25-hydroxycholesterol protects macrophages from cholesterol overload, thereby preventing mitochondrial DNA release and subsequent activation of the AIM2 inflammasome (By similarity).
See full target information CH25H
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搭配使用产品

Primary Antibodies

AB214295

Anti-CH25H antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CH25H antibody (AB214295)

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