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AB84717

重组人Cdk6蛋白

Recombinant Human Cdk6 protein

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(2 Publications)

Recombinant Human Cdk6 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB.

查看别名

CDKN6, CDK6, Cyclin-dependent kinase 6, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE

1 Images
SDS-PAGE - Recombinant Human Cdk6 protein (AB84717)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Cdk6 protein (AB84717)

SDS-PAGE of ab84717. Approximate MWt 39 kDa.

关键信息

纯度

>90% SDS-PAGE

表达系统

Baculovirus infected Sf9 cells

标签

His tag N-Terminus

应用

SDS-PAGE, WB

applications

生物活性

No

访问

Q00534

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q00534","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Cdk6 also known as Cyclin-dependent kinase 6 is a protein kinase involved in the regulation of the cell cycle. It has a molecular weight of approximately 40 kDa. Cdk6 is expressed in various tissues including lymphoid tissues and the brain. It plays a mechanical role by phosphorylating target proteins which impacts cell cycle progression particularly during the transition from G1 phase to S phase. Cdk6 can work in conjunction with D-type cyclins to form a complex that is critical for its activity.
Biological function summary

Cdk6 functions as a regulator of the cell cycle. It participates in a complex with cyclin D to control the G1 phase progression. This activity is essential for the regulation of cell division and proliferation. The association with cyclin D allows Cdk6 to phosphorylate the retinoblastoma protein (Rb) leading to the release of E2F transcription factors that promote the expression of genes necessary for DNA synthesis.

Pathways

Cdk6 is an important component of the cell cycle control pathway. It interacts primarily with the retinoblastoma protein and Cyclin D1 in this context. The cell cycle pathway is critical for controlled cell proliferation and its dysregulation can lead to diseases like cancer. Another involved pathway is the PI3K/AKT pathway which can activate Cdk6 activity through upstream signaling events implicating Cdk6 in cellular responses to growth signals.

Cdk6 has a significant role in cancer development due to its involvement in uncontrolled cell proliferation. Its overexpression or dysregulation is often linked to cancers such as leukemia and glioblastoma. In cancer the interaction with proteins like cyclin D1 can lead to unchecked progression through the cell cycle contributing to oncogenesis. Additionally Cdk6 inhibitors are being explored as therapeutic agents in cancer treatment due to their potential to restore control over cell cycle progression.
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特殊说明

形式

Liquid

附加说明

Purity >90% as determined by densitometry. Affinity purified.

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常规信息

功能

Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and negatively regulates cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed : 23918663).

序列相似性

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

翻译后修饰

Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and negatively regulates cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed : 23918663).
See full target information CDK6
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature aging 4:1231-1248 PubMed38951692

2024

Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Zumerle,Miles Sarill,Miriam Saponaro,Manuel Colucci,Liliana Contu,Edoardo Lazzarini,Roberta Sartori,Camilla Pezzini,Anna Rinaldi,Anna Scanu,Jacopo Sgrignani,Patrizia Locatelli,Marianna Sabbadin,Aurora Valdata,Daniela Brina,Isabella Giacomini,Beatrice Rizzo,Alessandra Pierantoni,Saman Sharifi,Silvia Bressan,Claudia Altomare,Yulia Goshovska,Chiara Giraudo,Roberto Luisetto,Luca Iaccarino,Cristina Torcasio,Simone Mosole,Emiliano Pasquini,Andrea Rinaldi,Laura Pellegrini,Gregorio Peron,Matteo Fassan,Stefano Masiero,Andrea Maria Giori,Stefano Dall'Acqua,Johan Auwerx,Pietro Cippà,Andrea Cavalli,Marco Bolis,Marco Sandri,Lucio Barile,Monica Montopoli,Andrea Alimonti

Oncology reports 45:706-716 PubMed33416185

2021

miRNA‑490‑3p promotes the metastatic progression of invasive ductal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Lu,Mei Zhang,Lu Lu,Yan-Zhao Liu,Hai-Hong Zhang,Xiao-Dong Liu
View all publications
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搭配使用产品

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

  • 0
  • 0
Proteins & Peptides

AB85247

Recombinant Human Cyclin D1 protein

SDS-PAGE - Recombinant Human Cyclin D1 protein (AB85247)

  • 0
  • 0
Primary Antibodies

AB124821

Anti-Cdk6 antibody [EPR4515]

KO Validated|RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 antibody [EPR4515] (AB124821)

  • 5
  • 2
Proteins & Peptides

AB119719

Recombinant Human Cyclin E1 protein

SDS-PAGE - Recombinant Human Cyclin E1 protein (AB119719)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

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