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重组人Cdk5蛋白(ab56282)

Reviews (1)Q&A (1)
SDS-PAGE - Recombinant Human Cdk5 protein (ab56282)
  • Sandwich ELISA - Recombinant Human Cdk5 protein (ab56282)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Tags: GST tag N-Terminus
  • Suitable for: SDS-PAGE

描述

  • 产品名称

    重组人Cdk5蛋白
    参阅全部 CDK5 蛋白酶

  • 纯度

    > 90 % SDS-PAGE.

  • 表达系统

    Escherichia coli

  • Accession

    Q00535

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Human

    • 预测分子量

      58 kDa

    • 标签

      GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab56282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 形式

    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

常规信息

  • 别名

    • Cdk 5
    • Cdk5
    • CDK5_HUMAN
    • Cell division protein kinase 5
    • Crk6
    • Cyclin dependent kinase 5
    • Cyclin-dependent kinase 5
    • Protein kinase CDK5 splicing
    • PSSALRE
    • Serine threonine protein kinase PSSALRE
    • Serine/threonine-protein kinase PSSALRE
    • Tau protein kinase II catalytic subunit
    • TPKII catalytic subunit
    see all

  • 功能

    Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.

  • 序列相似性

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.

  • 细胞定位

    Cytoplasm. Cell projection > lamellipodium. Cell projection > growth cone. In axonal growth cone with extension to the peripheral lamellipodia.
  • Information by UniProt

图片

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

发表研究结果有使用 ab56282？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab56282 尚未被引用在任何文献中。

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1-2 of 2 Abreviews or Q&A

Western blot Abreview for Cdk5 protein (Tagged)

 Excellent
Abreviews
abreview image
Application
Western blot

Review text: The blot image provided in this review has mouse Dorsal Striatum loaded at 70ug in lanes 2-7 and the CDK5-GST lysate in 1, 3 and 5 ug in lanes 8-10. The amount of CDK5-GST should be used at a 10th of that shown here, namely .1, .3 and .5 ug as the amount used was unnecessarily high. A goat polyclonal anti-GST antibody was used from GE Health sciences. Therefore, lanes 8-10 have a yellow band because of co-labeling of CDK5 and the GST tag. There was some nonspecific binding with the CDK5-GST lysate due to such a high amount being used. There was also nonspecific binding with the anti-GST antibody within the CDK5-GST lysate but not the Dorsal Striatum lysates.
Sample: Mouse Tissue lysate - whole (Dorsal Striatum)
Loading amount: 70 µg
Specification: Dorsal Striatum
Gel Running Conditions: Reduced Denaturing (10% gel)
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details
Incubation time: 18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk

Primary antibody (in addition to 'Cdk5 protein (Tagged)')
Name: Non-Abcam antibody was used: CDK5 (sc-173)
Host species: Rabbit
Clonality: Polyclonal
Dilution: 1/2000

Secondary antibody
Name: Non-Abcam antibody was used: Licor 700
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 700DX

Detection
Detection method: Licor
Exposure: 10 minute(s) and 0 second(s)
Bands: Specific: 32 and 56 kDa Non-specific: 37 kDa
Positive control: The CDK5-GST lysate was the positive control.
Negative control: None.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 30 2011

Question

Is this protein active? If not are there alternative Cdk5 proteins on the catalog which are active?

Read More
Answer

I just am writring to let you know that the active protein is on our catalog. The product ID is ab123653 and named p35 + p25 protein (Tagged).

Read More

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