重组人Cdk4 + Cyclin D1蛋白(ab55695)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies
描述
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产品名称
重组人Cdk4 + Cyclin D1蛋白 -
生物活性
Specific Activity is lot specific. Please contact our technical team to get lot specific information.
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纯度
> 75 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
标签
GST tag N-Terminus -
额外的序列信息
MW: Cdk4 = 57 kDa and CyclinD1 = 61 kDa.
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相关产品
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Related Products
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab55695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
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形式
Liquid -
补充说明
14nmol/min/mg using protein substrate Rb (773-928).ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.8766% Sodium chloride, 0.30732% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- B cell CLL lymphoma 1
- BCL 1 oncogene
- BCL1
see all -
相关性
CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase. Phosphorylation at Thr-172 is necessary for enzymatic activity. CDK4 mutations are involved in tumor formation, with defects in CDK4 resulting in cutaneous malignant melanoma 3 (CMM3). Cyclin D1 is essential for the control of the cell cycle at the G1/S transition. It interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. -
细胞定位
Nuclear
图片
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SDS-PAGE - Recombinant Human Cdk4 + Cyclin D1 protein (ab55695)SDS-PAGE analysis of ab55695 with molecular weight markers. Approximate molecular weights: 57kDa (CDK4)/61kDa (CyclinD1).
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Functional Studies - Recombinant Human Cdk4 + Cyclin D1 protein (ab55695)Kinase assay using ab55695 using protein substrate Rb. The specific activity of CDK4/CyclinD1 is 14 nmol/min/mg.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab55695 被引用在 6 文献中.
- McCann JL et al. The DNA deaminase APOBEC3B interacts with the cell-cycle protein CDK4 and disrupts CDK4-mediated nuclear import of Cyclin D1. J Biol Chem 294:12099-12111 (2019). PubMed: 31217276
- Wang Y et al. Design, synthesis and biological evaluation of pyrimidine derivatives as novel CDK2 inhibitors that induce apoptosis and cell cycle arrest in breast cancer cells. Bioorg Med Chem 26:3491-3501 (2018). PubMed: 29853338
- Wang H et al. The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival. Nature 546:426-430 (2017). PubMed: 28607489
- Kondo E et al. Tumour lineage-homing cell-penetrating peptides as anticancer molecular delivery systems. Nat Commun 3:951 (2012). PubMed: 22805558
- Cho HS et al. RB1 methylation by SMYD2 enhances cell cycle progression through an increase of RB1 phosphorylation. Neoplasia 14:476-86 (2012). PubMed: 22787429
- Nakajima K et al. Coordinated regulation of differentiation and proliferation of embryonic cardiomyocytes by a jumonji (Jarid2)-cyclin D1 pathway. Development 138:1771-82 (2011). PubMed: 21447557