重组人Cdc25C蛋白(ab105911)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人Cdc25C蛋白
参阅全部 Cdc25C 蛋白酶 -
生物活性
The Specific activity of ab105911 was determined to be 22 nmol/min/mg.
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纯度
> 70 % SDS-PAGE.
Purity was determined to be >70% by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
84 kDa including tags -
氨基酸
1 to 473 -
标签
GST tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab105911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- CDC 25
- Cdc 25C
- CDC25
see all -
功能
Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity. -
序列相似性
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain. -
发展阶段
Expressed predominantly in G2 phase. -
翻译后修饰
Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. -
细胞定位
Nucleus. - Information by UniProt
图片
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Functional Studies - Recombinant human Cdc25C protein (ab105911)The specific activity of Cdc25C (ab105911) was determined to be 22 phosphate released/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human Cdc25C protein (ab105911)SDS PAGE analysis of ab105911 -
SDS-PAGE - Recombinant human Cdc25C protein (ab105911)SDS PAGE analysis of ab105911 -
Functional Studies - Recombinant human Cdc25C protein (ab105911)Phosphatase Assay demonstrating specific activity of ab105911. -
SDS-PAGE - Recombinant human Cdc25C protein (ab105911)SDS-PAGE showing ab105911 at approximately 84kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab105911 尚未被引用在任何文献中。