重组人CD73蛋白(Active) (ab271449)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人CD73蛋白(Active)
参阅全部 CD73 蛋白酶 -
生物活性
Specific activity >20 nmol/mon/μg.
Assay was done in 50 µL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2 and 100 µM AMP for 12 min at 37º C. Inorganic phosphate produced was measured using Biomol Green reagent.
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纯度
>= 90 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
WELTILHTND VHSRLEQTSE DSSKCVNASR CMGGVARLFT KVQQIRRAEP NVLLLDAGDQ YQGTIWFTVY KGAEVAHFMN ALRYDAMALG NHEFDNGVEG LIEPLLKEAK FPILSANIKA KGPLASQISG LYLPYKVLPV GDEVVGIVGY TSKETPFLSN PGTNLVFEDE ITALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DVVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTSDDGRKVP VVQAYAFGKY LGYLKIEFDE RGNVISSHGN PILLNSSIPE DPSIKADINK WRIKLDNYST QELGKTIVYL DGSSQSCRFR ECNMGNLICD AMINNNLRHT DEMFWNHVSM CILNGGGIRS PIDERNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDLSRKPG DRVVKLDVLC TKCRVPSYDP LKMDEVYKVI LPNFLANGGD GFQMIKDELL RHDSGDQDIN VVSTYISKMK VIYPAVEGRI K -
预测分子量
59 kDa including tags -
分子量信息
Protein runs at higher than predicted MW on SDS-PAGE due to glycosylation. -
氨基酸
27 to 547 -
标签
His tag C-Terminus -
额外的序列信息
Genbank: NM_002526
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技术指标
Our Abpromise guarantee covers the use of ab271449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- 5' NT
- 5' nucleotidase (CD73)
- 5' nucleotidase precursor
see all -
功能
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
疾病相关
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
序列相似性
Belongs to the 5'-nucleotidase family. -
细胞定位
Cell membrane. - Information by UniProt
图片
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Functional Studies - Recombinant Human CD73 protein (Tagged) (ab271449)
Functional analysis of ab271449.
Specific activity >20 nmol/mon/μg.
Assay was done in 50 µL assay buffer containing 40 mM Tris, pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 40 µM AMP for 20 min at 37°C.
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SDS-PAGE - Recombinant Human CD73 protein (Tagged) (ab271449)SDS-PAGE analysis of ab271449.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271449 尚未被引用在任何文献中。