重组人 CD45 蛋白 (His-DDDDK tag N-Terminus)
Recombinant Human CD45 protein (His-DDDDK tag N-Terminus)
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Recombinant Human CD45 protein (His-DDDDK tag N-Terminus) is a Human Fragment protein, in the 1031 to 1251 aa range, expressed in Escherichia coli, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec.
查看别名
CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human CD45 protein (His-DDDDK tag N-Terminus) (AB177717)
15% SDS-PAGE analysis of ab177717 (3μg).
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human CD45 protein (His-DDDDK tag N-Terminus) (AB177717)
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
反应性数据
序列信息
性能和储存信息
运输条件
推荐的短期储存时间
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.
Pathways
Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.
特殊说明
形式
Liquid
附加说明
ab177717 was purified using conventional chromatography techniques.
常规信息
功能
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
序列相似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
翻译后修饰
Heavily N- and O-glycosylated.
靶点信息
搭配使用产品
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