重组人CD226蛋白(Active) (Biotin) (ab271541)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: Avi tag C-Terminus, Fc tag C-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人CD226蛋白(Active) (Biotin)
参阅全部 CD226 蛋白酶 -
生物活性
CD226, biotin binding to CD155. Briefly, 6 ng of CD226-biotin was mixed with varying amounts of CD155, His-tag in CD226 assay buffer at a final volume of 10 µL and allowed to incubate for 1 hr at room temp. Nickle acceptor beads were added for 30 mins, followed by Strep-conjugated acceptor beads for 30 mins and reading alpha-counts.
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纯度
>= 90 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
EEVLWHTSVPFAENMSLECVYPSMGILTQVEWFKIGTQQDSIAIFSPTHG MVIRKPYAERVYFLNSTMASNNMTLFFRNASEDDVGYYSCSLYTYPQGTW QKVIQVVQSDSFEAAVPSNSHIVSEPGKNVTLTCQPQMTWPVQAVRWEKI QPRQIDLLTYCNLVHGRNFTSKFPRQIVSNCSHGRWSVIVIPDVTVSDSG LYRCYLQASAGENETFVMRLTVAEGKTDN -
预测分子量
55 kDa -
氨基酸
19 to 247 -
标签
Avi tag C-Terminus , Fc tag C-Terminus -
额外的序列信息
Fc portion of human IgG1.
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偶联物
Biotin
技术指标
Our Abpromise guarantee covers the use of ab271541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
补充说明
Enzymatically biotin-labeled using Avi-tag™ technology
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- adhesion glycoprotein
- CD226
- CD226 antigen
see all -
功能
Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell. -
组织特异性
Expressed by peripheral blood T-lymphocytes. -
序列相似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
细胞定位
Membrane. - Information by UniProt
图片
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Functional Studies - Recombinant human CD226 protein (Active) (Biotin) (ab271541)Specific activity of ab271541.
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SDS-PAGE - Recombinant human CD226 protein (Active) (Biotin) (ab271541)SDS-PAGE analysis of 2 µg ab271541.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271541 尚未被引用在任何文献中。