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AB271541

重组人CD226蛋白(Active) (Biotin)

Recombinant human CD226 protein (Active) (Biotin)

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Recombinant human CD226 protein (Active) (Biotin) is a Human Fragment protein, in the 19 to 247 aa range, expressed in HEK 293 cells, with >90%, suitable for SDS-PAGE, FuncS.

查看别名

CD226, DNAM1, CD226 antigen, DNAX accessory molecule 1, DNAM-1

2 Images
Functional Studies - Recombinant human CD226 protein (Active) (Biotin) (AB271541)
  • FuncS

Supplier Data

Functional Studies - Recombinant human CD226 protein (Active) (Biotin) (AB271541)

Specific activity of ab271541.

SDS-PAGE - Recombinant human CD226 protein (Active) (Biotin) (AB271541)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human CD226 protein (Active) (Biotin) (AB271541)

SDS-PAGE analysis of 2 μg ab271541.

关键信息

纯度

>90% SDS-PAGE

表达系统

HEK 293 cells

标签

Fc tag C-Terminus Avi tag C-Terminus

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

CD226, biotin binding to CD155. Briefly, 6 ng of CD226-biotin was mixed with varying amounts of CD155, His-tag in CD226 assay buffer at a final volume of 10 μL and allowed to incubate for 1 hr at room temp. Nickle acceptor beads were added for 30 mins, followed by Strep-conjugated acceptor beads for 30 mins and reading alpha-counts.

偶联物

Biotin

激发波长/发射波长
访问

Q15762

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.4 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Enzymatically biotin-labeled using Avi-tag™ technology
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序列信息

[{"sequence":"EEVLWHTSVPFAENMSLECVYPSMGILTQVEWFKIGTQQDSIAIFSPTHGMVIRKPYAERVYFLNSTMASNNMTLFFRNASEDDVGYYSCSLYTYPQGTWQKVIQVVQSDSFEAAVPSNSHIVSEPGKNVTLTCQPQMTWPVQAVRWEKIQPRQIDLLTYCNLVHGRNFTSKFPRQIVSNCSHGRWSVIVIPDVTVSDSGLYRCYLQASAGENETFVMRLTVAEGKTDN","proteinLength":"Fragment","predictedMolecularWeight":"54.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":247,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q15762","tags":[{"tag":"Fc","terminus":"C-Terminus"},{"tag":"Avi","terminus":"C-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
储存信息
Avoid freeze / thaw cycle|Store in the dark
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD226 also known as DNAM-1 (DNAX Accessory Molecule-1) is a transmembrane protein expressed on the surface of various immune cells such as T cells natural killer (NK) cells and some B cells. The protein has a molecular weight of approximately 65 kDa. CD226 is part of the Ig superfamily and plays a role in the adhesion and signaling processes of immune cells. It interacts with its ligands CD112 (PVRL2) and CD155 (PVR) on target cells facilitating immune synapse formation and cytotoxic activity.
Biological function summary

CD226 is involved in immune cell activation and receptor-mediated signaling. It aids the recognition and destruction of target cells by NK cells and cytotoxic T lymphocytes through its interaction with ligand-expressing cells. CD226 does not work alone but functions as part of a complex system of co-stimulatory and inhibitory receptors balancing immune responses. Its cooperation with receptors such as CD96 and TIGIT (T cell immunoreceptor with Ig and ITIM domains) modulates the activity of immune effector cells.

Pathways

CD226 takes part in immune signaling pathways that regulate cell-mediated cytotoxicity and inflammation. It is an important component of the immune checkpoint axis interacting closely with proteins like CD96 and TIGIT within these pathways. This interaction helps determine the fate of immune responses influencing the progression or resolution of inflammatory and autoimmune conditions. CD226 plays a role in the NK cell-mediated cytotoxicity pathway and the adaptive immune response.

CD226 has been linked to autoimmune diseases such as multiple sclerosis and type 1 diabetes. Abnormal expression or function of CD226 can influence the pathogenesis of these conditions often through interactions with related proteins like TIGIT impacting immune tolerance and effector cell function. CD226's involvement in regulating immune responses highlights its importance as a potential therapeutic target in managing autoimmune and other immune-related disorders.
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特殊说明

形式

Liquid

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常规信息

功能

Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed : 8673704, PubMed : 9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed : 26755705, PubMed : 31253644, PubMed : 30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).

翻译后修饰

PKC-mediated phosphorylation at Ser-329 is required for lipid raft recruitment (PubMed:15684041). Phosphorylation of Tyr-322 requires association with lipid rafts and allows GRB2 binding and activation of subsequent signaling (By similarity).

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产品实验方案

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靶点信息

Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed : 8673704, PubMed : 9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed : 26755705, PubMed : 31253644, PubMed : 30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).
See full target information CD226
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