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AB290112

重组人CD14蛋白(Active)

Recombinant Human CD14 Protein (Active)

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Recombinant Human CD14 Protein (Active) is a Human Full Length protein, in the 20 to 352 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for HPLC, Mass Spec, SDS-PAGE, FuncS.

查看别名

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

4 Images
Functional Studies - Recombinant Human CD14 Protein (Active) (AB290112)
  • FuncS

Lab

Functional Studies - Recombinant Human CD14 Protein (Active) (AB290112)

Fully biologically active determined by its ability to enhance LPS-induced IL-8 secretion in THP-1 human acute monocytic leukemia cells. ED50 is ≤ 49.2 ng/ml, corresponding to a specific activity of 2.03 x 10^4 units/mg.

Mass Spectrometry - Recombinant Human CD14 Protein (Active) (AB290112)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human CD14 Protein (Active) (AB290112)

Mass determination by ESI-TOF. Predicted MW is 35830.06 Da (+/-10 Da by ESI-TOF). Observed MW is 35834.28 Da.

SDS-PAGE - Recombinant Human CD14 Protein (Active) (AB290112)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human CD14 Protein (Active) (AB290112)

SDS-PAGE analysis of ab290112

HPLC - Recombinant Human CD14 Protein (Active) (AB290112)
  • HPLC

Supplier Data

HPLC - Recombinant Human CD14 Protein (Active) (AB290112)

HPLC analysis of ab290112

关键信息

纯度

>95% HPLC

SDS-PAGE >= 95%

内毒素水平

<0.005 EU/µg

表达系统

HEK 293 cells

标签

Tag free

应用

HPLC, Mass Spec, FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

Fully biologically active determined by its ability to enhance LPS-induced IL-8 secretion in THP-1 human acute monocytic leukemia cells.

ED50 is ≤ 49.2 ng/ml, corresponding to a specific activity of 2.03 x 104 units/mg.

访问

P08571

不含动物源

Yes

不含载体蛋白

No

种属

Human

复溶

Reconstitute in PBS

存储溶液

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Proteins & Peptides

AB177661

Recombinant Human HLA-DR protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human HLA-DR protein (His tag N-Terminus) (AB177661)

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  • 0
Proteins & Peptides

AB271801

Recombinant Human CD16a (mutated F176V) protein (Avi tag C-Terminus + His tag C-Terminus)

SDS-PAGE - Recombinant Human CD16a (mutated F176V) protein (Avi tag C-Terminus + His tag C-Terminus) (AB271801)

  • 0
  • 0
Proteins & Peptides

AB158776

Recombinant Human CD11c protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human CD11c protein (GST tag N-Terminus) (AB158776)

  • 0
  • 0
Primary Antibodies

AB183322

Anti-CD14 antibody [SP192]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (AB183322)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"TTPEPCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVDADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQWLKPGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERGLMAALCPHKFPAIQNLALRNTGMETPTGVCAALAAAGVQPHSLDLSHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLRVLDLSCNRLNRAPQPDELPEVDNLTLDGNPFLVPGTALPHEGSMNSGVVPAC","proteinLength":"Full Length","predictedMolecularWeight":"35.83 kDa","actualMolecularWeight":"35.83 kDa","aminoAcidEnd":352,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P08571","tags":[]}]
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性能和储存信息

形式
Lyophilized
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
Ambient
推荐的长期储存条件
Ambient
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.
Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.
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常规信息

功能

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).

翻译后修饰

N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

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产品实验方案

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靶点信息

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).
See full target information CD14
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