重组人CD134 / OX40L receptor蛋白(Fc Chimera) (ab83676)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人CD134 / OX40L receptor蛋白(Fc Chimera)
参阅全部 CD134 / OX40L receptor 蛋白酶 -
纯度
> 95 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
Theoretical sequence: LHCVGDTYPSNDRCCHECRPGNGMVSRCSRSQNTVCRPC GPGFYNDVVSSKPCKP CTWCNLRSGSERKQLCTATQDTVCRCRAGTQP LDSYKPGVDCAPCPPGHFSPGDNQ ACKPWTNCTLAGKHTLQPASNSSD AICEDRDPPATQPQETQGPPARPITVQPTEAWP RTSQGPSTRPVGIPK VDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMIS RTPE VTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTV LHQ DWLNGKEYKCRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRD ELTKNQVSLTCLV KGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSF FLYSKLTVDKSRWQQGNVFSCS VMHEALHNHYTQKSLSLSPGK -
氨基酸
1 to 207 -
标签
Fc tag C-Terminus -
额外的序列信息
Fused with the Fc region of Human IgG1 at the C-terminus.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab83676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 1% Human serum albumin, 10% Trehalose
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复溶It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
常规信息
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别名
- ACT 35
- ACT35
- ACT35 antigen
see all -
功能
Receptor for TNFSF4/OX40L/GP34. -
序列相似性
Contains 4 TNFR-Cys repeats. -
细胞定位
Membrane. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human CD134 / OX40L receptor protein (Fc Chimera) (ab83676)Lane 1 MW markers; Lane 2 ab83676; Lane 3 ab83676 treated with PNGase F to remove potential N-linked glycans; Lane 4 ab83676 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 μg protein loaded per lane; Deep Purple™ stained.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Slight drop in MW after treatment with glycosidase cocktail suggests presence of Olinked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes. -
SDS-PAGE - Recombinant Human CD134 / OX40L receptor protein (Fc Chimera) (ab83676)A sample of ab83676 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris HCl 2D gel. 40 μg protein loaded per lane; Deep Purple™ stained. Spot train indicates presence of multiple isoforms of the Chimera. Spots within the spot train were cut from the gel and identified as the Chimera by protein mass fingerprinting. -
Functional Studies - Recombinant Human CD134 / OX40L receptor protein (Fc Chimera) (ab83676)Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified ab83676 exists in multiple isoforms, which differ according to their level of post-translational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins.
Triangle indicates theoretical pI and MW of the protein.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab83676 尚未被引用在任何文献中。
