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AB323128

Recombinant Human Cathepsin C Protein Standard (His tag)

Recombinant Human Cathepsin C Protein Standard (His tag)

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Recombinant Human Cathepsin C Protein Standard (His tag) is a Human Full Length protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.

查看别名

CPPI, CTSC, Dipeptidyl peptidase 1, Cathepsin C, Cathepsin J, Dipeptidyl peptidase I, Dipeptidyl transferase, DPP-I, DPPI

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Sandwich ELISA - Recombinant Human Cathepsin C Protein Standard (His tag) (AB323128)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Cathepsin C Protein Standard (His tag) (AB323128)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human Cathepsin C Protein Standard (His tag) (AB323128)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Cathepsin C Protein Standard (His tag) (AB323128)

SDS-PAGE analysis of ab323128 under reducing conditions for 2ug protein.

关键信息

纯度

>80% SDS-PAGE

表达系统

HEK 293 cells

标签

His tag C-Terminus

应用

SDS-PAGE, sELISA

applications

生物活性

No

访问

P53634

不含动物源

Yes

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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序列信息

[{"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"51.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P53634","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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特殊说明

形式

Liquid

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常规信息

功能

Thiol protease (PubMed : 1586157). Has dipeptidylpeptidase activity (PubMed : 1586157). Active against a broad range of dipeptide substrates composed of both polar and hydrophobic amino acids (PubMed : 1586157). Proline cannot occupy the P1 position and arginine cannot occupy the P2 position of the substrate (PubMed : 1586157). Can act as both an exopeptidase and endopeptidase (PubMed : 1586157). Activates serine proteases such as elastase, cathepsin G and granzymes A and B (PubMed : 8428921).

序列相似性

Belongs to the peptidase C1 family.

翻译后修饰

N-glycosylated. While glycosylation at Asn-53, Asn-119 and Asn-276 is mediated by STT3A-containing complexes, glycosylation at Asn-29 is mediated STT3B-containing complexes.. In approximately 50% of the complexes the exclusion domain is cleaved at position 58 or 61. The two parts of the exclusion domain are held together by a disulfide bond.

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产品实验方案

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靶点信息

Thiol protease (PubMed : 1586157). Has dipeptidylpeptidase activity (PubMed : 1586157). Active against a broad range of dipeptide substrates composed of both polar and hydrophobic amino acids (PubMed : 1586157). Proline cannot occupy the P1 position and arginine cannot occupy the P2 position of the substrate (PubMed : 1586157). Can act as both an exopeptidase and endopeptidase (PubMed : 1586157). Activates serine proteases such as elastase, cathepsin G and granzymes A and B (PubMed : 8428921).
See full target information CTSC
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搭配使用产品

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

  • 5
  • 1

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