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重组人Carbonic Anhydrase 9/CA9蛋白(ab114200)

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SDS-PAGE - Recombinant Human Carbonic Anhydrase 9/CA9 protein (ab114200)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, SDS-PAGE, WB

    描述

    • 产品名称

      重组人Carbonic Anhydrase 9/CA9蛋白
      参阅全部 Carbonic Anhydrase 9/CA9 蛋白酶

    • 表达系统

      Wheat germ

    • Accession

      Q16790

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 序列

        MAPLCPSPWLPLLIPAPAPGLTVQLLLSLLLLMPVHPQRLPRMQEDSPLG GGSSGEDDPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKP KSEEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWP RVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGH SVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEI HVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIA EEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVM LSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRA AEPVQLNSCLAAGDILALVFGLLFAVTSVAFLVQMRRQHRRGTKGGVSYR PAEVAETGA

      • 预测分子量

        77 kDa including tags

      • 氨基酸

        1 to 459

    技术指标

    Our Abpromise guarantee covers the use of ab114200 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      ELISA

      SDS-PAGE

      Western blot

    • 形式

      Liquid
    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    常规信息

    • 别名

      • CA-IX
      • CA9
      • CAH9_HUMAN
      • CAIX
      • Carbonate dehydratase IX
      • Carbonic anhydrase 9
      • Carbonic anhydrase IX
      • Carbonic dehydratase
      • G250
      • Membrane antigen MN
      • MN
      • P54/58N
      • pMW1
      • RCC associated protein G250
      • RCC-associated antigen G250
      • Renal cell carcinoma-associated antigen G250
      see all

    • 功能

      Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

    • 组织特异性

      Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

    • 序列相似性

      Belongs to the alpha-carbonic anhydrase family.
      Contains 1 alpha-carbonic anhydrase domain.

    • 翻译后修饰

      Asn-346 bears high-mannose type glycan structures.

    • 细胞定位

      Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
    • Information by UniProt

    图片

    • SDS-PAGE - Recombinant Human Carbonic Anhydrase 9/CA9 protein (ab114200)
      SDS-PAGE - Recombinant Human Carbonic Anhydrase 9/CA9 protein (ab114200)
      12.5% SDS-PAGE showing ab114200 at approximately 76.56kDa stained with Coomassie Blue.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab114200？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab114200 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    Hello, I am writing you re your Indirect ELISA kits and hope you can advise which kit buy and protocol to use. We have developed RCC mouse model using  RENCA mouse RCC cells over expressing the human CAIX protein. We would like to test/control for the possible presence of mouse anti human CAIX antibodies in the mouse sera. That is to say we would like to coat the plates with recombinant human CAIX protein, use OUR mice sera as primary Abs (as a “detector Antibodies”) and then follow with a secondary Anti Mouse Ab  HRP Conjugated/other detection conjugate. Could you possibly advise which kit buy and protocol to use. Thank you

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    Answer

    Thank you for contacting us. Unfortunately we do not have any kit corresponding to the detection of the CAIX protein. However, I would suggest to develop an ELISA assay using the CAIX protein ab114200 that would be coated on the plate. A control anti-CAIX, such as ab15086, for which the concentration is known, may be used to create a standard curve and to quantify the amount of anti-CAIX antibodies contained in the mouse sera. ab15086 is a rabbit antibody. A mouse antibody would ideal, unfortunately, the only mouse anti-CAIX we have (ab107257) is not purified. It is an ascites surpernatant and the concentration has therefore not been determined. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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