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AB123189

重组人 CANT1 蛋白 (His tag N-Terminus)

Recombinant Human CANT1 protein (His tag N-Terminus)

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Recombinant Human CANT1 protein (His tag N-Terminus) is a Human Fragment protein, in the 63 to 401 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

查看别名

SHAPY, CANT1, Soluble calcium-activated nucleotidase 1, SCAN-1, Apyrase homolog, Putative MAPK-activating protein PM09, Putative NF-kappa-B-activating protein 107

1 Images
SDS-PAGE - Recombinant Human CANT1 protein (His tag N-Terminus) (AB123189)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CANT1 protein (His tag N-Terminus) (AB123189)

15% SDS-PAGE showing ab123189 (3μg).

关键信息

纯度

>90% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

Mass Spec, SDS-PAGE

applications

生物活性

No

访问

Q8WVQ1

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.29% Sodium chloride, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMRPAPGRPPTHNAHNWRLGQAPANWYNDTYPLSPPQRTPAGIRYRIAVIADLDTESRAQEENTWFSYLKKGYLTLSDSGDKVAVEWDKDHGVLESHLAEKGRGMELSDLIVFNGKLYSVDDRTGVVYQIEGSKAVPWVILSDGDGTVEKGFKAEWLAVKDERLYVGGLGKEWTTTTGDVVNENPEWVKVVGYKGSVDHENWVSNYNALRAAAGIQPPGYLIHESACWSDTLQRWFFLPRRASQERYSEKDDERKGANLLLSASPDFGDIAVSHVGAVVPTHGFSSFKFIPNTDDQIIVALKSEEDSGRVASYIMAFTLDGRFLLPETKIGSVKYEGIEFI","proteinLength":"Fragment","predictedMolecularWeight":"40.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":401,"aminoAcidStart":63,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8WVQ1","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CANT1 also known as calcium-activated nucleotidase 1 acts as a nucleotidase enzyme that removes phosphate groups from nucleotides. It catalyzes the hydrolysis of UDP ADP and GDP to their respective monophosphates. The protein of CANT1 has an approximate molecular mass of 41 kDa. This protein is widely distributed throughout human tissues with higher expression levels observed in skeletal muscle and heart.
Biological function summary

CANT1 influences cellular processes by regulating extracellular nucleotide signaling. It involves in protein glycosylation a process critical for protein folding and stability. CANT1 does not form part of a complex but functions independently to exert its biological effects. Glycosylation impacts how proteins function and signal across the body reflecting the importance of CANT1's enzymatic role in various cells.

Pathways

Multiple cellular pathways depend on CANT1’s enzymatic activity. The protein is notably engaged in the purinergic signaling pathway where it modulates extracellular nucleotide levels that influence signaling cascades. CANT1 shares functional relationships with proteins such as ENTPD5 and NTPDases which partake in similar nucleotide hydrolysis processes. It also has connections to metabolic processes within glucose metabolism.

Aberrations in CANT1's function link to diseases like Desbuquois dysplasia and specific forms of osteochondrodysplasia. In these conditions defects in glycosylation arise due to CANT1 mutations and affect bone growth and development. This protein also interacts with proteins involved in bone disorders including SLC35D1 contributing to the pathology observed in affected patients.

特殊说明

形式

Liquid

附加说明

ab123189 is purified using conventional chromatography techniques.

常规信息

功能

Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP (PubMed : 12234496, PubMed : 15006348, PubMed : 15248776, PubMed : 16835225). Involved in proteoglycan synthesis (PubMed : 22539336).

序列相似性

Belongs to the apyrase family.

翻译后修饰

N-glycosylated.

产品实验方案

靶点信息

Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP (PubMed : 12234496, PubMed : 15006348, PubMed : 15248776, PubMed : 16835225). Involved in proteoglycan synthesis (PubMed : 22539336).
See full target information CANT1

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