重组人CaMKII beta蛋白(ab132961)
Key features and details
- Expression system: Wheat germ
- Purity: > 99% Purified via GST Tag
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB, ELISA, Functional Studies
描述
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产品名称
重组人CaMKII beta蛋白
参阅全部 CaMKII beta 蛋白酶 -
纯度
> 99 % Purified via GST Tag. -
表达系统
Wheat germ -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKL SARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFE DIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKG AAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWAC GVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLIN QMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKL KGAILTTMLATRNFSAAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPP AALESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDP GLTSFEPEALGNLVEGMDFHRFYFENLLAKNSKPIHTTILNPHVHVIGED AACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWQNVHFHCSGAPVA PLQ -
预测分子量
81 kDa including tags -
氨基酸
1 to 503 -
标签
GST tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab132961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
ELISA
Functional Studies
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形式
Liquid -
补充说明
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HClThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Calcium/calmodulin dependent protein kinase (CaM kinase) II beta
- Calcium/calmodulin dependent protein kinase II beta
- Calcium/calmodulin dependent protein kinase IIB
see all -
功能
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
组织特异性
Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. - Information by UniProt
图片
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12.5% SDS-PAGE analysis of ab132961 stained with Coomassie Blue.
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Kinase Assay using ab132961.
Fig.1: SDS-PAGE gel
Fig.2: Autoradiography of SDS-PAGE gel.
Kinase substrates:
H3 histone, family 3A - calf thymus - Molecular weight: approx. 16.5-17 kDa on SDS-PAGE
Myelin basic protein - bovine brain - Molecular weight: approx. 21-22 kDa on SDS-PAGE
Histone H1.0 - calf thymus - Molecular weight: approx. 32-33 kDa on SDS-PAGE
Beta-casein protein- bovine milk - Molecular weight: approx. 33-34 kDa on SDS-PAGE
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab132961 尚未被引用在任何文献中。