Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

重组人CaMKII alpha蛋白(ab60899)

Submit a review Q&A (3)References (4)
Functional Studies - Recombinant human CaMKII alpha protein (ab60899)
  • SDS-PAGE - Recombinant human CaMKII alpha protein (ab60899)
  • Functional Studies - Recombinant human CaMKII alpha protein (ab60899)
  • SDS-PAGE - Recombinant human CaMKII alpha protein (ab60899)
  • Western blot - Recombinant human CaMKII alpha protein (ab60899)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 80% Densitometry
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE, WB

描述

  • 产品名称

    重组人CaMKII alpha蛋白

  • 生物活性

    256 nmol/min/mg

  • 纯度

    > 80 % Densitometry.
    Affinity purified.

  • 表达系统

    Baculovirus infected Sf9 cells

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Human

    • 序列

      MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEF MATITCTRFT EEYQLFEELG KGAFSVVRRC VKVLAGQEYA AKIINTKKLS ARDHQKLERE ARICRLLKHP NIVRLHDSIS EEGHHYLIFD LVTGGELFED IVAREYYSEA DASHCIQQIL EAVLHCHQMG VVHRDLKPEN LLLASKLKGA AVKLADFGLA IEVEGEQQAW FGFAGTPGYL SPEVLRKDPY GKPVDLWACG VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK MLTINPSKRI TAAEALKHPW ISHRSTVASC MHRQETVDCL KKFNARRKLK GAILTTMLAT RNFSGGKSGG NKKSDGVKES SESTNTTIED EDTKVRKQEI IKVTEQLIEA ISNGDFESYT KMCDPGMTAF EPEALGNLVE GLDFHRFYFE NLWSRNSKPV HTTILNPHIH LMGDESACIA YIRITQYLDA GGIPRTAQSE ETRVWHRRDG KWQIVHFHRS GAPSVLPH

    • 预测分子量

      74 kDa

    • 标签

      GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab60899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    SDS-PAGE

    Western blot

  • 形式

    Liquid

  • 补充说明

    ab188554 (CaMKII peptide) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名

    • Alpha CaMKII
    • Calcium calmodulin dependent protein kinase II
    • Calcium/calmodulin dependent protein kinase II alpha B subunit
    • Calcium/calmodulin dependent protein kinase type II alpha chain
    • Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
    • Calcium/calmodulin-dependent protein kinase II alpha
    • Calcium/calmodulin-dependent protein kinase II-alpha
    • Calcium/calmodulin-dependent protein kinase type II subunit alpha
    • Calcium/calmodulin-dependent protein kinase type IIA
    • CaM kinase II alpha chain
    • CaM kinase II alpha subunit
    • CaM kinase II subunit alpha
    • CaMK II alpha subunit
    • CaMK-II subunit alpha
    • Camk2a
    • CAMKA
    • CaMKII
    • CaMKIINalpha
    • EC 2.7.11.17
    • KCC2A_HUMAN
    • KIAA0968
    • MGC123320
    • MGC139375
    • MGC155201
    • mKIAA0968
    • PK2CDD
    • PKCCD
    • R74975
    • zgc:112538
    • zgc:123320
    see all

  • 功能

    CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.

  • 序列相似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.

  • 细胞定位

    Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
  • Information by UniProt

图片

  • Functional Studies - Recombinant human CaMKII alpha protein (ab60899)
    Functional Studies - Recombinant human CaMKII alpha protein (ab60899)
    The specific activity of CaMKII alpha (ab60899) was determined to be 295 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human CaMKII alpha protein (ab60899)
    SDS-PAGE - Recombinant human CaMKII alpha protein (ab60899)
    SDS PAGE analysis of ab60899
  • Functional Studies - Recombinant human CaMKII alpha protein (ab60899)
    Functional Studies - Recombinant human CaMKII alpha protein (ab60899)
    Sample Kinase Activity Plot.
  • SDS-PAGE - Recombinant human CaMKII alpha protein (ab60899)
    SDS-PAGE - Recombinant human CaMKII alpha protein (ab60899)
    ab60899 on SDS-PAGE, MW ~74 kDa.
  • Western blot - Recombinant human CaMKII alpha protein (ab60899)
    Western blot - Recombinant human CaMKII alpha protein (ab60899)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (4)

发表研究结果有使用 ab60899？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab60899 被引用在 4 文献中.

  • Chen Y  et al. Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance. EMBO Rep 23:e53373 (2022). PubMed: 34994492
  • Li H  et al. β-hydroxybutyrate reduces reinstatement of cocaine conditioned place preference through hippocampal CaMKII-α β-hydroxybutyrylation. Cell Rep 41:111724 (2022). PubMed: 36450263
  • Marimoutou M  et al. Oxidation of Methionine 77 in Calmodulin Alters Mouse Growth and Behavior. Antioxidants (Basel) 7:N/A (2018). PubMed: 30322141
  • Yuen EY  et al. Restoration of glutamatergic transmission by dopamine D4 receptors in stressed animals. J Biol Chem 288:26112-20 (2013). PubMed: 23884421

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-3 of 3 Abreviews or Q&A

Question

Could you provide the protocol and buffers used to verify this protein's activity?

Read More
Answer

For the activity assay the lab followed the following procedure:

1. 10 ul of diluted ab60899

2. 7.5 ul of 1 mg/ml substrate (Autocamtide 2 synthetic peptide substrate (KKALRRQETVDAL-amide) diluted in dH2O

3. 2.5 ul of Ca2+/Calmodulin Solution, 10X (formulation below):

10X Ca2+/Calmodulin solution:
10ng/ul Calmodulin, 4mM CaCl2, 10mM Tris-HCl, pH 7.2

Read More

Question

Is this protein active in the absence of Calcium?

Read More
Answer

ab60899 has not been modified in any way to make it permanently active in the absence of calcium. However, it is possible that this protein, like other CAMK2a proteins, can autophosphorylate itself in the absence of Ca2+/Calmodulin, making it active, so it is possible that it can activate itself in the absence of Ca2+/Calmodulin.
In their activity testing of ab60899, the laboratory did add a Ca2+/Calmodulin solution to activate ab60899.

Read More

Question

Hi, I am interested in ELISA kit for the human CAMKII alpha (ab2724) . There is no ready to use kit and I wonder if you can help me to choose anitbodies for ELISA sandwich. I have already use the two of your products for Western Blot. ab22609 and ab 2724 that is my target and ab60899 for quantifieng the western blot. I would realy appreciate if you help me to choose the antibodies for running Sandwich ELISA and the plates that need for runing my expereiments. Best Regards

Read More
Answer

Thank you for contacting us. Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere with the other antibody binding. Therefore, we are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested for sandwich ELISA. At this moment we do not have tested CamKII antibody pairs that can be used in sELISA. However based on the requirement of different epitope specificity I can recommend using ab54925 or ab54926 with ab89197 or ab92332. The antibodies are specific to different part of antigens so these can be used in sELISA. Please be advised that sELISA is not a tested application so these products are not covered under Abpromise guarantee. However I can certainly offer you Abreview discount codes as these products are eligible. The discount codes will give you next primary antibody free of charge if you test these antibodies in sELISA and submit an Abreview. You may have to use one of these antibodies as detection or capture so I can provide you 2 discount codes for 2 products. Let me know if you are interested in obtaining this offer I will then be sending you the discount codes right away. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com