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AB60899

重组人CaMKII alpha蛋白

Recombinant human CaMKII alpha protein

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(4 Publications)

Recombinant human CaMKII alpha protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB, FuncS.

查看别名

CAMKA, KIAA0968, CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha

5 Images
Functional Studies - Recombinant human CaMKII alpha protein (AB60899)
  • FuncS

Unknown

Functional Studies - Recombinant human CaMKII alpha protein (AB60899)

Sample Kinase Activity Plot.

Functional Studies - Recombinant human CaMKII alpha protein (AB60899)
  • FuncS

Unknown

Functional Studies - Recombinant human CaMKII alpha protein (AB60899)

The specific activity of CaMKII alpha (ab60899) was determined to be 295 nmol/min/mg as per activity assay protocol

Western blot - Recombinant human CaMKII alpha protein (AB60899)
  • WB

Unknown

Western blot - Recombinant human CaMKII alpha protein (AB60899)

All lanes:

Anti-CaMKII alpha antibody (<a href='/products/unavailable/camkii-alpha-antibody-ab75930'>ab75930</a>) at 1/100 dilution

All lanes:

Western blot - Recombinant human CaMKII alpha protein (ab60899) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 10s

SDS-PAGE - Recombinant human CaMKII alpha protein (AB60899)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CaMKII alpha protein (AB60899)

ab60899 on SDS-PAGE, MW ~74 kDa.

SDS-PAGE - Recombinant human CaMKII alpha protein (AB60899)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CaMKII alpha protein (AB60899)

SDS PAGE analysis of ab60899

关键信息

纯度

>80% Densitometry

表达系统

Baculovirus infected Sf9 cells

标签

GST tag N-Terminus

应用

WB, FuncS, SDS-PAGE

applications

生物活性

Yes

生物学活性

256 nmol/min/mg

访问

Q9UQM7

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab60899 can be used as a WB positive control in conjunction with <a href='/products/unavailable/camkii-alpha-antibody-ab75930'>ab75930</a>.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Specific Activity: 932 nmol/min/mg.</p>" } } }
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产品详情

ab188554 (CaMKII peptide) can be utilized as a substrate for assessing Kinase activity
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序列信息

[{"linker":null,"sequence":"MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSPEFMATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWISHRSTVASCMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSGGKSGGNKKSDGVKESSESTNTTIEDEDTKVRKQEIIKVTEQLIEAISNGDFESYTKMCDPGMTAFEPEALGNLVEGLDFHRFYFENLWSRNSKPVHTTILNPHIHLMGDESACIAYIRITQYLDAGGIPRTAQSEETRVWHRRDGKWQIVHFHRSGAPSVLPH","proteinLength":"Full Length","predictedMolecularWeight":"74 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9UQM7","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CaMKII alpha also known as CAMK2A is an enzyme belonging to the CaMK kinase family. This protein is a calcium/calmodulin-dependent protein kinase and exhibits a molecular weight of approximately 50 to 60 kDa. CaMKII alpha is primarily expressed in the brain specifically in the hippocampus and other regions involved in synaptic activity. Due to its role as a serine/threonine kinase CaMKII alpha is significant in phosphorylating various substrates as part of cellular signaling.
Biological function summary

CaMKII alpha plays an important role in synaptic plasticity a process vital for learning and memory. The protein acts as an important component of the postsynaptic density complex where it interacts with other proteins to regulate synaptic strength. When calcium levels rise CaMKII alpha is activated through phosphorylation altering its conformation and allowing interactions with downstream substrates. This regulatory mechanism highlights its role in modulating neurotransmission synaptic potentiation and memory consolidation.

Pathways

CaMKII alpha functions within the calcium signaling pathway contributing to long-term potentiation (LTP) and long-term depression (LTD). It interacts with proteins such as NMDA receptors to facilitate changes in synaptic strength alongside other kinases. This pathway's activation leads to memory formation and synaptic plasticity in the central nervous system. Additionally CaMKII alpha connects with the MAPK signaling pathway integrating signals from various neurotransmitter systems which help coordinate neuronal response to stimuli.

Alterations in CaMKII alpha have been linked to neurological conditions such as Alzheimer's disease and schizophrenia. In Alzheimer's discourse in CaMKII alpha phosphorylation correlates with impaired memory and cognitive function. The protein also interfaces with tau another key protein related to neurofibrillary tangles in Alzheimer's. In schizophrenia abnormalities in CaMKII alpha expression and activity suggest its role in synaptic dysfunction and neurodevelopmental irregularities. Targeting CaMKII alpha may provide therapeutic opportunities for managing these and related neurological disorders.
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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed : 14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed : 28130356). Also regulates the migration of developing neurons (PubMed : 29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed : 23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed : 11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed : 35568036). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of PSAT1, inhibiting ferroptosis by promoting GPX4 hydroxylation and stability (PubMed : 40281343). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).

序列相似性

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

翻译后修饰

Autophosphorylation of Thr-286 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state.. Palmitoylated. Probably palmitoylated by ZDHHC3 and ZDHHC7.

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产品实验方案

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靶点信息

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed : 14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed : 28130356). Also regulates the migration of developing neurons (PubMed : 29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed : 23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed : 11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed : 35568036). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of PSAT1, inhibiting ferroptosis by promoting GPX4 hydroxylation and stability (PubMed : 40281343). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).
See full target information CAMK2A
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 41:111724 PubMed36450263

2022

β-hydroxybutyrate reduces reinstatement of cocaine conditioned place preference through hippocampal CaMKII-α β-hydroxybutyrylation.

Applications

Unspecified application

Species

Unspecified reactive species

Hongchun Li,Xuemei Wan,Zhixiang Wu,Yuanyi Zhou,Rong Chen,Wei Xu,Jiamei Zhang,Zhen Yang,Lin Bai,Jie Zhang,Feng Qin,Liang Wang,Yaxing Chen,Linhong Jiang,Yuman He,Xiaojie Wang,Qingfan Wei,Shu Li,Yanping Dai,Yuanyuan Chen,Yonghai Wang,Hongbo Wang,Jingwei Tian,Yinglan Zhao,Xiaobo Cen

EMBO reports 23:e53373 PubMed34994492

2022

Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Chen,Xiao Wu,Jing Zhang,Guopin Pan,Xiaoyun Wang,Xiaosun Guo,Jianli Wang,Xiaopei Cui,Haiqing Gao,Mei Cheng,Jingwen Yang,Cheng Zhang,Fan Jiang

Antioxidants (Basel, Switzerland) 7: PubMed30322141

2018

Oxidation of Methionine 77 in Calmodulin Alters Mouse Growth and Behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Méry Marimoutou,Danielle A Springer,Chengyu Liu,Geumsoo Kim,Rodney L Levine

The Journal of biological chemistry 288:26112-20 PubMed23884421

2013

Restoration of glutamatergic transmission by dopamine D4 receptors in stressed animals.

Applications

Unspecified application

Species

Unspecified reactive species

Eunice Y Yuen,Ping Zhong,Xiangning Li,Jing Wei,Zhen Yan
View all publications
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搭配使用产品

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

  • 5
  • 1
Proteins & Peptides

AB84552

Recombinant human CaMKII delta protein

Functional Studies - Recombinant human CaMKII delta protein (AB84552)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Biochemicals

AB120192

Dynasore, dynamin inhibitor

Immunocytochemistry/ Immunofluorescence - Dynasore, dynamin inhibitor (AB120192)

  • 0
  • 0
Primary Antibodies

AB2900

Anti-EEA1 antibody - Early Endosome Marker

KO Validated|Lab Essentials

Western blot - Anti-EEA1 antibody - Early Endosome Marker (AB2900)

  • 4
  • 39

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