重组人CAMK1G蛋白(ab51400)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
-
产品名称
重组人CAMK1G蛋白
参阅全部 CAMK1G 蛋白酶 -
生物活性
Specific activity was determined to be 788 nmol/min/mg.
-
纯度
> 90 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
氨基酸
1 to 330 -
标签
GST tag N-Terminus
-
技术指标
Our Abpromise guarantee covers the use of ab51400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
SDS-PAGE
Functional Studies
-
形式
Liquid -
补充说明
ab188554 (CaMKII peptide) can be utilized as a substrate for assessing Kinase activity
This product was previously labelled as CaMKI gamma
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- Calcium/calmodulin dependent protein kinase type 1G
- Calcium/calmodulin-dependent protein kinase I-gamma
- Calcium/calmodulin-dependent protein kinase Ig
see all -
功能
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1. -
组织特异性
Mainly expressed in brain with small amounts in skeletal muscles, kidney, spleen and liver. Strongly expressed in forebrain neocortex, striatum and limbic system. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
结构域
The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate. -
翻译后修饰
May be prenylated on Cys-473. -
细胞定位
Cytoplasm. Golgi apparatus membrane. Cell membrane. - Information by UniProt
图片
-
Functional Studies - Recombinant human CAMK1G protein (ab51400)The specific activity of CAMK1G (ab51400) was determined to be 675 nmol/min/mg as per activity assay protocol
-
SDS-PAGE - Recombinant human CAMK1G protein (ab51400)SDS PAGE analysis of ab51400 -
Functional Studies - Recombinant human CAMK1G protein (ab51400)Kinase activity assay. Specific activity 788 nmol/min/mg. -
SDS-PAGE - Recombinant human CAMK1G protein (ab51400)Recombinant human CAMK1G (with proprietary tag)
molecular weight 69 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab51400 尚未被引用在任何文献中。