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AB56280

重组人c-Fos蛋白

Recombinant Human c-Fos protein

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(2 Publications)

Recombinant Human c-Fos protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS, WB.

查看别名

G0S7, FOS, Protein c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, Proto-oncogene c-Fos, Transcription factor AP-1 subunit c-Fos

3 Images
Western blot - Recombinant Human c-Fos protein (AB56280)
  • WB

Project

Western blot - Recombinant Human c-Fos protein (AB56280)

ab56280, c-Fos protein (Tagged), has a predicted molecular weight of 78 kDa in SDS-PAGE.

All lanes:

Western blot - Anti-c-Fos (phospho T232) antibody (<a href='/products/primary-antibodies/c-fos-phospho-t232-antibody-ab43175'>ab43175</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human c-Fos protein (ab56280) at 0.1 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 41 kDa

Observed band size: 78 kDa

false

Western blot - Recombinant Human c-Fos protein (AB56280)
  • WB

Lab

Western blot - Recombinant Human c-Fos protein (AB56280)

All lanes:

Anti-c-Fos antibody - ChIP Grade (<a href='/products/unavailable/c-fos-antibody-chip-grade-ab7963'>ab7963</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human c-Fos protein (ab56280) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 20s

SDS-PAGE - Recombinant Human c-Fos protein (AB56280)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human c-Fos protein (AB56280)

SDS-PAGE analysis of ab56280 with molecular weight markers. Approximate molecular weight 78kDa due to presence of a Tag.

关键信息

纯度

>90% SDS-PAGE

表达系统

Baculovirus infected Sf9 cells

标签

GST tag N-Terminus

应用

WB, SDS-PAGE, FuncS

applications

生物活性

No

访问

P01100

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Kinsae assay</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab56280 can be used as a WB positive control in conjunction with <a href='/products/unavailable/c-fos-antibody-chip-grade-ab7963'>ab7963</a>. Shows a band detected around 75 kDa, this higher size due to the tag on the protein.</p>" } } }
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序列信息

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P01100","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The c-Fos protein also known as c-Fos is a component of the Fos family of transcription factors which includes FosB Fra-1 and Fra-2. These proteins play a role in regulating gene expression. c-Fos has a molecular weight of about 55 kDa. You usually find c-Fos expressed in various tissues including the brain providing a marker for neuronal activity and is often induced in response to stimuli like stress growth factors and mitogens. In research settings c-Fos staining and c-Fos immunohistochemistry are techniques commonly used to study activation patterns in cells and tissues.
Biological function summary

C-Fos acts as part of the Activator Protein-1 (AP-1) complex a group of DNA-binding proteins that regulate gene transcription. This complex forms when c-Fos and Jun proteins dimerize. The AP-1 complex controls several cellular processes such as proliferation differentiation and apoptosis. Due to its participation in these vital processes c-Fos influences cellular responses to external stimuli and can affect how cells behave under stress.

Pathways

C-Fos significantly contributes to the MAPK/ERK pathway which plays a role in cell growth and differentiation. It partners with Jun proteins to form functional transcription factors within the MAPK signaling cascade. Additionally c-Fos is involved in the JNK signaling pathway linking it to stress responses and apoptosis. These pathways highlight the partnerships between c-Fos and other proteins like ERK and JNK indicating their intertwined roles in cellular signaling networks.

Researchers associate c-Fos with cancer and neurodegenerative diseases. In cancer c-Fos expression correlates with changes in AP-1 activity which can lead to altered cell growth and proliferation potentially contributing to tumor formation. Its involvement in neurodegenerative diseases reveals its role in neuron apoptosis and cellular stress responses. In these scenarios proteins like Bcl-2 also play a part with CDK1 sharing connections in processes that affect cell cycle and apoptosis illustrating c-Fos's broad significance in both normal and pathological conditions.
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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

序列相似性

Belongs to the bZIP family. Fos subfamily.

翻译后修饰

Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation (By similarity).. Constitutively sumoylated with SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.. In quiescent cells, the small amount of FOS present is phosphorylated at Tyr-10 and Tyr-30 by SRC. This Tyr-phosphorylated form is cytosolic. In growing cells, dephosphorylated by PTPN2. Dephosphorylation leads to the association with endoplasmic reticulum membranes and activation of phospholipid synthesis.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
See full target information FOS
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 44:108 PubMed40148973

2025

C-FOS promotes the formation of neutrophil extracellular traps and the recruitment of neutrophils in lung metastasis of triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Yan,Wenxi Zhao,Juntong Du,Lizhi Teng,Tong Yu,Peng Xu,Jiangnan Liu,Ru Yang,Yuhan Dong,Hongyue Wang,Lingran Lu,Weiyang Tao

eLife 12: PubMed39786853

2025

A new pipeline SPICE identifies novel JUN-IKZF1 composite elements.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Li,Sree Pulugulla,Sonali Das,Jangsuk Oh,Rosanne Spolski,Jian-Xin Lin,Warren J Leonard
View all publications
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搭配使用产品

Primary Antibodies

AB134122

Anti-c-Fos antibody [EPR883(2)]

KO Validated|Recombinant|RabMAb|Lab Essentials

Flow Cytometry (Intracellular) - Anti-c-Fos antibody [EPR883(2)] (AB134122)

  • 1
  • 3
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB50036

Recombinant human TGF beta 1 protein (Active)

Bioactive

  • 0
  • 0
Proteins & Peptides

AB268982

Recombinant Human STAT3 protein (His tag)

SDS-PAGE - Recombinant Human STAT3 protein (His tag) (AB268982)

  • 0
  • 0

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