重组人Brk/PTK6蛋白(ab60888)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人Brk/PTK6蛋白
参阅全部 Brk/PTK6 蛋白酶 -
纯度
> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as Brk
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Breast tumor kinase
- Breast tumour kinase
- Protein tyrosine kinase 6
see all -
功能
Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression. -
组织特异性
Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻译后修饰
Autophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme. -
细胞定位
Cytoplasm. Nucleus. Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors. - Information by UniProt
图片
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Functional Studies - Recombinant human Brk/PTK6 protein (ab60888)The specific activity of Brk/PTK6 (ab60888) was determined to be 136 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human Brk/PTK6 protein (ab60888)SDS PAGE analysis of ab60888 -
SDS-PAGE - Recombinant human Brk/PTK6 protein (ab60888)SDS PAGE analysis of ab60888 -
Functional Studies - Recombinant human Brk/PTK6 protein (ab60888)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human Brk/PTK6 protein (ab60888)ab60888 on SDS-PAGE, MW ~80 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab60888 被引用在 1 文献中.
- Miczi M et al. Identification of Host Cellular Protein Substrates of SARS-COV-2 Main Protease. Int J Mol Sci 21:N/A (2020). PubMed: 33333742