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AB201428

重组人BRAF (mutated K601E)蛋白

Recombinant human BRAF (mutated K601E) protein

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Recombinant human BRAF (mutated K601E) protein is a Human Fragment protein, in the 416 to 766 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, FuncS.

查看别名

BRAF1, RAFB1, BRAF, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1

2 Images
Functional Studies - Recombinant human BRAF (mutated K601E) protein (AB201428)
  • FuncS

Supplier Data

Functional Studies - Recombinant human BRAF (mutated K601E) protein (AB201428)

Specific activity of ab201428 as determined by kinase assay

SDS-PAGE - Recombinant human BRAF (mutated K601E) protein (AB201428)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human BRAF (mutated K601E) protein (AB201428)

SDS PAGE analysis of ab201428

关键信息

纯度

>80% SDS-PAGE

表达系统

Baculovirus infected Sf9 cells

标签

GST tag N-Terminus

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

Specific activity 120 nmol/ min/ mg

访问

P15056

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.02% PMSF, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"63 kDa","actualMolecularWeight":null,"aminoAcidEnd":766,"aminoAcidStart":416,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P15056","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
储存信息
Avoid freeze / thaw cycle
True
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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).

序列相似性

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

翻译后修饰

Phosphorylation at Ser-365 by SGK1 inhibits its activity (Ref.8, PubMed:11410590). Phosphorylation at Thr-753 by MAPK1 (PubMed:19710016). Dephosphorylation of Ser-365 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC); this relieves inactivation and stimulates kinase activity (PubMed:35768504, PubMed:35830882).. Methylation at Arg-671 decreases stability and kinase activity.. Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).
See full target information BRAF mutated K601E
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