Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE
参阅全部 BMP4 蛋白酶
The ED50 as determined by dose-dependent induction of alkaline phosphatase activity with C2C12 cells is in the range of 2-12μg/ml.
纯度> 95 % SDS-PAGE.
Sterile filtered. Endotoxin level is < 0.1 ng per µg of BMP4.
蛋白长度Full length protein
氨基酸293 to 408
Our Abpromise guarantee covers the use of ab51998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is manufactured by BioVision, an Abcam company and was previously called 4578 BMP-4, human recombinant. 4578-10 is the same size as the 10 µg size of ab51998.
The lyophilized protein is best-stored desiccated below 0oC. Reconstituted BMP-4 should be stored in working aliquots at -20oC. For long-term storage, it is recommended to add a carrier protein (0.1% BSA). Following reconstitution, this solution can be diluted into other aqueous buffers and stored at 4oC for 1 week or –20oC for future use.
Endotoxin level is < 0.1 ng per µg.
Concentration information loading...
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 10.269% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- BMP 2B
- BMP 4
功能Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
组织特异性Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
疾病相关Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero.
序列相似性Belongs to the TGF-beta family.
细胞定位Secreted > extracellular space > extracellular matrix.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.