重组人BLK蛋白(ab60876)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人BLK蛋白
参阅全部 BLK 蛋白酶 -
纯度
> 90 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- B lymphocyte kinase
- B lymphoid tyrosine kinase
- Blk
see all -
功能
Modulator of beta-cells function, acting through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. -
组织特异性
Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles. -
疾病相关
Defects in BLK are a cause of maturity-onset diabetes of the young type 11 (MODY11) [MIM:613375]. MODY11 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. - Information by UniProt
图片
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Functional Studies - Recombinant human BLK protein (ab60876)The specific activity of BLK (ab60876) was determined to be 110 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human BLK protein (ab60876)SDS PAGE analysis of ab60876 -
SDS-PAGE - Recombinant human BLK protein (ab60876)SDS PAGE analysis of ab60876 -
Functional Studies - Recombinant human BLK protein (ab60876)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human BLK protein (ab60876)ab60876 on SDS-PAGE, MW ~84 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab60876 被引用在 1 文献中.
- Ichikawa-Tomikawa N et al. The Src-Family Kinases SRC and BLK Contribute to the CLDN6-Adhesion Signaling. Cells 12:N/A (2023). PubMed: 37443730