AB180062

重组人Bcl-XL蛋白(His tag)

Recombinant Human Bcl-XL protein (His tag)

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Recombinant Human Bcl-XL protein (His tag) is a Human Fragment protein, in the 1 to 212 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

查看别名

BCL2L, BCLX, BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X

1 Images

SDS-PAGE - Recombinant Human Bcl-XL protein (His tag) (AB180062)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Bcl-XL protein (His tag) (AB180062)

SDS-PAGE of reduced ab180062 stained under reducing (R) condition overnight with Coomassie Blue. The purity of the protein is greater than 95%.

关键信息

纯度

>95% SDS-PAGE

内毒素水平

< 1 EU/µg

表达系统

Escherichia coli

应用

SDS-PAGE

applications

生物活性

访问

Q07817

不含动物源

不含载体蛋白

种属

Human

复溶

Reconstitute at 200 µg/mL in water

存储溶液

pH: 7.4 Constituents: 5% Trehalose, 0.48% HEPES, 0.37% Potassium chloride

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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序列信息

[{"sequence":"MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNR","proteinLength":"Fragment","predictedMolecularWeight":"24.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":212,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q07817","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

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性能和储存信息

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-XL also known as Bcl-xL or Bcl-Xl protein is a member of the Bcl-2 protein family playing an important role in regulating cell death. Bcl-XL inhibits apoptosis by binding to pro-apoptotic proteins preventing the release of cytochrome c from mitochondria. This protein has a molecular weight of approximately 30 kDa. Bcl-xL is widely expressed in tissues notably in the brain endothelial cells and hematopoietic tissues where it contributes to cell survival.

Biological function summary

This protein acts as an anti-apoptotic molecule within the mitochondria and is part of the Bcl-2 family complex. Bcl-XL modulates the balance between pro-apoptotic and anti-apoptotic signals impacting cell fate. By binding and sequestering pro-apoptotic members such as Bax and Bak Bcl-XL prevents mitochondrial outer membrane permeabilization important for avoiding programmed cell death. This function not only supports cellular longevity but also understanding cancer cell survival.

Pathways

The Bcl-XL protein holds a significant position in the apoptotic signaling pathways. It is particularly involved in the intrinsic pathway where its interactions with mitochondrial proteins Bax and Bak determine cell survival or death. It is interconnected with the PI3K/AKT pathway where AKT kinase activity can upregulate Bcl-XL expression demonstrating how survival signals are transmitted. These interactions highlight its pivotal role in balancing life and death at the cellular level.

Bcl-XL's overexpression often connects to the progression of cancers and resistance to chemotherapy. By inhibiting programmed cell death Bcl-XL allows cancer cells to evade traditional treatments. Furthermore research implicates Bcl-XL in neurodegenerative disorders where its interaction with Bcl-2 proteins disrupts normal apoptotic processes contributing to cellular dysfunction and disease. Understanding Bcl-XL's disease-related roles furthers the development of targeted therapies combating its protective effects in pathological contexts.

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特殊说明

形式

Lyophilized

附加说明

Purified by Immobilized metal affinity chromatography.

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常规信息

功能

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).. Isoform Bcl-X(S) promotes apoptosis.

序列相似性

Belongs to the Bcl-2 family.

翻译后修饰

Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.. Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis.. Ubiquitinated by RNF183 during prolonged ER stress, leading to degradation by the proteosome.

亚细胞定位

Mitochondrion inner membrane

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information BCL2L1

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重组人Bcl-XL蛋白(His tag)

Recombinant Human Bcl-XL protein (His tag)

SDS-PAGE - Recombinant Human Bcl-XL protein (His tag) (AB180062)

关键信息

纯度

内毒素水平

表达系统

标签

应用

生物活性

访问

不含动物源

不含载体蛋白

种属

复溶

存储溶液