重组人ATP1B1蛋白(Active) (ab222961)
Key features and details
- Expression system: Baculovirus infected Sf21 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人ATP1B1蛋白(Active)
参阅全部 ATP1B1 蛋白酶 -
生物活性
Specific activity is > 3,000 pmol/min/µg, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-triphosphate to phosphate per minute per minute at pH 7.5 at 25°C.
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纯度
> 90 % SDS-PAGE.
Affinity purified -
内毒素水平
< 1.000 Eu/µg -
表达系统
Baculovirus infected Sf21 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
ADPEFKPTYQ DRVAPPGLTQ IPQIQKTEIS FRPNDPKSYE AYVLNIVRFL EKYKDSAQRD DMIFEDCGDV PSEPKERGDF NHERGERKVC RFKLEWLGNC SGLNDETYGY KEGKPCIIIK LNRVLGFKPK PPKNESLETY PVMKYNPNVL PVQCTGKRDE DKDKVGNVEY FGLGNSPGFP LQYYPYYGKL LQPKYLQPLL AVQFTNLTMD TEIRIECKAY GENIGYSEKD RFQGRFDVKI EVKSHHHHHH -
预测分子量
29 kDa including tags -
氨基酸
63 to 303 -
标签
His tag C-Terminus -
额外的序列信息
Extracellular domain.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab222961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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补充说明
Background reference: PMID 26506237.
Shi et al. Overexpression of ATP1B1 predicts an adverse prognosis in cytogenetically normal acute myeloid leukemia. Oncotarget. 2016 Jan 19;7(3):2585-95. doi: 10.18632/oncotarget.6226.
This product was previously labelled as beta 1 Sodium Potassium ATPase
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 10% Glycerol (glycerin, glycerine), PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Adenosinetriphosphatase
- AT1B1_HUMAN
- ATP 1B
see all -
功能
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. -
组织特异性
Found in most tissues. -
序列相似性
Belongs to the X(+)/potassium ATPases subunit beta family. -
细胞定位
Membrane. - Information by UniProt
图片
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SDS-PAGE - Recombinant human ATP1B1 protein (Active) (ab222961)
SDS-PAGE - Recombinant human ATP1B1 protein (Active) (3 µg ab222961).
28-40 KDa under reducing conditions.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab222961 尚未被引用在任何文献中。