重组人ATG16L1蛋白(ab132520)
Key features and details
- Expression system: Wheat germ
- Suitable for: SDS-PAGE, WB, ELISA
描述
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产品名称
重组人ATG16L1蛋白 -
表达系统
Wheat germ -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MAQLRIKHQEELTELHKKRGELAQLVIDLNNQMQRKDREMQMNEAKIAEC LQTISDLETECLDLRTKLCDLERANQTLKDEYDALQITFTALEGKLRKTT EENQELVTRWMAEKAQEANRLNAENEKDSRRRQARLQKELAEAAKEPLPV EQDDDIEVIVDETSDHTEETSPVRAISRAATKRLSQPAGGLLDSITNIFG RRSVSSFPVPQDNVDTHPGSGKEVRVPATALCVFDAHDGEVNAVQFSPGS RLLATGGMDRRVKLWEVFGEKCEFKGSLSGSNAGITSIEFDSAGSYLLAA SNDFASRIWTVDDYRLRHTLTGHSGKVLSAKFLLDNARIVSGSHDRTLKL WDLRSKVCIKTVFAGSSCNDIVCTEQCVMSGHFDKKIRFWDIRSESIVRE MELLGKITALDLNPERTELLSCSRDDLLKVIDLRTNAIKQTFSAPGFKCG SDWTRVVFSPDGSYVAAGSAEGSLYIWSVLTGKVEKVLSKQHSSSINAVA WSPSGSHVVSVDKGCKAVLWAQY -
预测分子量
85 kDa including tags -
氨基酸
85 to 607
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技术指标
Our Abpromise guarantee covers the use of ab132520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
ELISA
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8
常规信息
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别名
- A16L1_HUMAN
- APG16 like 1
- APG16-like 1
see all -
功能
Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane. -
疾病相关
Inflammatory bowel disease 10 -
序列相似性
Belongs to the WD repeat ATG16 family.
Contains 7 WD repeats. -
翻译后修饰
Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli. -
细胞定位
Cytoplasm. Preautophagosomal structure membrane. Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human ATG16L1 protein (ab132520)12.5% SDS-PAGE analysis of ab132520 stained with Coomassie Blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab132520 尚未被引用在任何文献中。
