重组人ARK5蛋白(ab125626)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
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产品名称
重组人ARK5蛋白 -
生物活性
The specific activity of ab125626 was determined to be 50 nmol/min/mg.
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纯度
> 80 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
122 kDa including tags -
氨基酸
1 to 661
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab125626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- AMP activated protein kinase family member 5
- AMPK related protein kinase 5
- AMPK-related protein kinase 5
see all -
功能
Involved in tolerance to glucose starvation. Phosphorylates ATM. Suppresses Fas-induced apoptosis by phosphorylation of CASP6, thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR. -
组织特异性
Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X. - Information by UniProt
图片
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SDS-PAGE - Recombinant human ARK5 protein (ab125626)SDS PAGE analysis of ab125626
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Functional Studies - Recombinant human ARK5 protein (ab125626)The specific activity of ARK5 (ab125626) was determined to be 54 nmol /min/mg as per activity assay protocol.
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Functional Studies - Recombinant human ARK5 protein (ab125626)The specific activity of ab125626 was determined to be 50 nmol/min/mg. -
SDS-PAGE - Recombinant human ARK5 protein (ab125626)SDS-PAGE analysis of ab125626.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125626 尚未被引用在任何文献中。